Clinical Chemistry Lab Exam with correct answers 2024
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Course
Clinical Chemistry Lab Ex
Institution
Clinical Chemistry Lab Ex
Wavelength Accuracy correct answers A physical characteristic of light radiation which specifies its color or frequency
*The correctness with which the wavelength of light striking the sample matches wavelength on instrument
Absorbance Accuracy correct answers Correctness with which a measure...
Clinical Chemistry Lab Exam 1
Wavelength Accuracy correct answers A physical characteristic of light radiation which
specifies its color or frequency
*The correctness with which the wavelength of light striking the sample matches
wavelength on instrument
Absorbance Accuracy correct answers Correctness with which a measured absorbance
matches true absorbance
Linearity correct answers A spectrophotometer is linear when any change in intensity of
light at detector will cause proportional change in measured output
Stray Light correct answers Any light received by detector of a spectrophotometer that
is outside the wavelength limits of the monochromator bandwidth
Bandwidth correct answers The width of the peak halfway between the top and the
bottom of the peak
QC for Wavelength correct answers 361 and 536 nm (daily)
QC for Absorbance correct answers 340 and 550 nm (daily)
QC for Linearity correct answers 550 nm (weekly)
QC for Stray Light correct answers 340 and 680 nm (weekly)
QC for Bandwidth correct answers 350-370 nm (weekly)
____ is one of the most advanced of a line of spectrometers. correct answers Gilford
Stasar III
The Stasar utilizes _____, ____, ____, ____, _____, and ____ (6) correct answers -
Tungsten light source
- Diffraction grating monochromator
- Linear absorbance photometer
- Four-place LED display
- Rapid Sampling system
- Temp controlled cuvette
The light source is a tungsten lamp that has spectral range of _____. correct answers
335-700 nm
, The diffraction grating monochromator provides an ______ and a wavelength accuracy
of ___. correct answers - 8 nm bandwidth
- (+/- 2 nm)
The flow-through cuvette is constructed of ____ and is impervious to ____. correct
answers - inert materials
- most solvents
A dual calibration standard containing a ____ and ____ filter is also provided and
permits wavelength calibration at ___ and ___ and absorbance verification at ___ and
___. correct answers - holmium oxide
- Inconel density
- 361
- 536
- 340
- 550
Calculate the mean correct answers (Add all the numbers) / (Total number of samples)
Calculate Standard Deviation correct answers - Find variance first
- (x-mean)squared / (n-1) = Variance
- Take square root of Variance
Calculate Coefficient of Variation correct answers Standard Deviation / mean X 100
What are 3 important variables that affect the accuracy of pipetting? correct answers 1)
Wetting the pipette
2) Tearing the weight
3) Wiping the pipette after collecting sample
If a pipet was expected to deliver 500 ul and only delivered 495 ul, what would be the
percentage of error? correct answers (495 ul - 500 ul) / 500 ul X 100
What is the dilution factor if 200 ul of sample is mixed with 1000 ul of diluent? correct
answers (200 ul)/(200 ul) : (1000ul + 200 ul) / 200 ul
= 1:6
How much diluent would you add to 100 ul of sample to make a X 5 dilution? correct
answers 5 = (100 ul + X) / 100 ul
Answer: 400 ul
How much sample would you add to 10 ml of diluent to make a X 5 dilution? correct
answers 5 = (X + 10 ml) / X
Answer: 2500 ml
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