MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES
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Course
MICR 351
Institution
MICR 351
MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES
Northern Hybridization? - Answer-mRNA-cDNA hybridization to find gene of interest using DNA probe to identify which bond holds gene of interest
Western Hybridization? - Answer-Antibody-protein hybridization, where antibodies bind...
MICR 351 TESTED QUESTIONS
WITH ALL ANSWERS AND ALL
PRINTED PAGES
Northern Hybridization? - Answer-mRNA-cDNA hybridization to find gene of interest
using DNA probe to identify which bond holds gene of interest
Western Hybridization? - Answer-Antibody-protein hybridization, where antibodies bind
to a specific protein bond that contains target protein that produces antibody
- used in vaccines and other tests, such as pregnancy tests
Functional genomics - Answer-Microarrays and RNA-sequence that rely on ability to
make short, single stranded DNA
What helps to create short, single stranded DNA? - Answer-Oligonucleotides or primer
synthesis
What is site directed mutagenesis? - Answer-Altering of a specific amino acid by
altering base changes on DNA/gene leading to proteins with altered activities
What technique can be used to amplify multiple copies of genes from few copies using
two primers? - Answer-Polymerase Chain Reaction with 2 oligonucleotides
What are the tree steps per PCR cycle? - Answer-1. Denature at 94ºC
2. Anneal at 50-60ºC
3. Extension at 68-72ºC
What type of PCR is more accurate? Real-time or reverse transcriptase? - Answer-
Real-time PCR
What is the difference between Sangerʻs dideoxy sequencing vs. dyedeoxy
sequencing? - Answer-Sangerʻs Dideoxy:
- one primer that anneals and extends
- build-up of sequence
- dideoxy nucleotide lacks 2ʻ an 3ʻOH, needs a 3ʻ OH to add on next base on
Dyedeoxy:
- includes color dye to dye each base a specific color
- reads 100 bases only
- takes a long time
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