MCB 2610 Lab Practical Exam Questions And Correct Answers.
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Course
MCB 2610
Institution
MCB 2610
MCB 2610 Lab Practical Exam Questions
And Correct Answers.
What is the process of contamination control? - answerAseptic technique
What are pipettes - answercalibrated glass tubes used to measure and dispense small volumes
of liquids
What color is the bigger vs. smaller micropipettors pipettes...
MCB 2610 Lab Practical Exam Questions
And Correct Answers.
What is the process of contamination control? - answer✔✔Aseptic technique
What are pipettes - answer✔✔calibrated glass tubes used to measure and dispense small volumes
of liquids
What color is the bigger vs. smaller micropipettors pipettes? - answer✔✔Green - bigger
Blue - smaller
What is the purpose of isolating bacteria? - answer✔✔To establish a PURE CULTURE, to be
able to study to study the physiology and morphology of a particular bacterial type
What is the quickest and most economical method of bacterial isolation? - answer✔✔Streak
plate method
What must you use to view items under 100x? - answer✔✔immersion oil
What does parfocal mean? - answer✔✔It means that if you focus the specimen in one objective,
it'll be in approximate focus in all objectives
What is a simple stain? - answer✔✔Staining procedures that only use one stain
What is a direct stain? - answer✔✔A simple stain that colors the bacteria
What is a negative stain? - answer✔✔Simple stain that stains the background but leaves the
bacteria unstained
What is the purpose of heat fixing? - answer✔✔1. Denaturing bacterial enzymes to prevent
autolysis
2. Enhances the adherence of bacterial cells to microscope slide
In Gram stains, why can't Gram negative bacteria retain the crystal violet? - answer✔✔Because
Gram negative bacteria have an outer membrane and a thinner layer of peptidoglycan, which are
easily depolarized and can only retain the counterstain (Safranin-pink)
What are the 3 most common stain? - answer✔✔Gram, capsule, and spore stain
The ion that is colored contains a chromophore group
What is it called if the chromophore is a positive ion (like methylene blue)? - answer✔✔Basic
stain
What is it called if the chromophore is a negative ion? - answer✔✔Acidic stain
What causes endospore formation? - answer✔✔It's a response to certain environmental
conditions such as depletion of nutrients, heating, drying, and other unfavorable conditions.
Heat used during staining does not cause sporulationn
Procedure for gram stain - answer✔✔flood violet - 1 min
WASH
flood iodine - 1 min
WASH
drip alcohol - 15-30 sec
WASH
Counterstain safranin - 30-40
Dye used for spore staining - answer✔✔5% aqueous Malachite green dye
How is microbial growth defined? - answer✔✔Increase in population size
What are the 4 stages of the growth curve of microbial growth? - answer✔✔lag, log, stationary,
death
Lag phase - answer✔✔Occurs when the bacteria is transferred to a fresh growth medium. The
cells must adjust to new conditions and synthesize a full complement of enzymes.
Log phase - answer✔✔Period of tremendous growth in population. Each cell divides into two
new cells; two-fold exponential growth
Stationary phase - answer✔✔When a culture is grown in a closed vessel, it will eventually use
up all the limiting nutrients and may produce waste products in sufficient quantities to curtail
growth
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