How does SYBR-Green stain DNA? - CORRECT ANSWER -It binds to the minor groves
of the DNA
If a large air bubble is allowed to set in the agarose? - CORRECT ANSWER -It will obscure
results
if the electrodes were reversed? - CORRECT ANSWER -The DNA would run off
backwards
if the gel is run...
BSC2010L CH13 Lab Quiz Review Questions and
Correct Answers Graded Highest 100% A++
How does SYBR-Green stain DNA? - CORRECT ANSWER ✔✔ -It binds to the minor groves
of the DNA
If a large air bubble is allowed to set in the agarose? - CORRECT ANSWER ✔✔ -It will obscure
results
if the electrodes were reversed? - CORRECT ANSWER ✔✔ -The DNA would run off
backwards
if the gel is run at very high voltage? - CORRECT ANSWER ✔✔ -The DNA will run off
If too much DNA is loaded in the well? - CORRECT ANSWER ✔✔ -It will overflow and skew
the results
Is it necessary to use a fresh tip when delivering the same reagent? - CORRECT ANSWER ✔✔
-avoid contamination
What are two functions of the loading dye? - CORRECT ANSWER ✔✔ --Track the migration
-Add density to the sample
What is a restriction enzyme? - CORRECT ANSWER ✔✔ -An enzyme that cuts DNA at a
specific sequence of nucleotides
What percentage was the agarose gel which you ran? - CORRECT ANSWER ✔✔ -.8%
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