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Microbiology 250 Lab Practical Exam Questions and Complete Solutions Graded A+ $14.49   Add to cart

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Microbiology 250 Lab Practical Exam Questions and Complete Solutions Graded A+

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  • Course
  • Microbiology 2104
  • Institution
  • Microbiology 2104

Microbiology 250 Lab Practical Exam Questions and Complete Solutions Graded A+

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  • August 26, 2024
  • 35
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Microbiology 2104
  • Microbiology 2104
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Microbiology 250 Lab
Practical Exam Questions
and Complete Solutions
Graded A+

Denning [Date] [Course title]

,brom cresol purple - Answer: indicator in MIO media that tests for decarboxylation of ornithine



positive result is a lavender-purple color



A



hydrogen peroxide - Answer: H2O2



used to test for organism's possession of catalase



copious amounts of bubbles are evidence for release of O2 as peroxide is broken down



2H2O2 + O2



iodine - Answer: indicates the presence or absence of starch



agar - Answer: solidifying agent purified from marine algae



colonial morphology - Answer: size, color, shape, elevation, consistency, form, margin, surface, optical
characteristics



should only be observed on isolated colonies



YA10SC - Answer: yeast agar loaded with 10% sucrose (table sugar)



encourages the growth of streptococcus organisms such as Streptococcus salivarius and Streptococcus
mutans



dental caries - Answer: result of bacterial fermentation of sugar, commonly sucrose

,Streptococcus mutans and Lactobacillus acidophilus are main microbes responsible for production of
acid that causes these



demineralization - Answer: as sugars are fermented an acid is made that dissolves the hard calcium layer
of enamel of the tooth



dental plaque - Answer: dense mass of bacteria



dental caries are formed under this



difficult to remove



microbes involved in advanced tooth decay - Answer: Actinomyces



Nocardia



fermentation - Answer: the chemical action of yeast on sugars



ubiquitous - Answer: term referring to the fact that microorganisms are found virtually everywhere



SMA plate - Answer: standard methods agar



used to assess viable bacterial growth of a sample



microscopic morphology - Answer: also known as cellular morphology



aids in the observation of microbial traits such as a cell shape, size, arrangement, stain reactions,
endospores

, primary shapes of bacteria - Answer: rod - Bacillus



sphere - coccus



spiral - Spirillum



parfocal - Answer: property of microscope which allows objectives to be changed without having to
refocus



refraction - Answer: the bending of light as it passes from one material to another



immersion oil - Answer: addition of this minimizes the amount of light that is refracted or lost



size of coccus cell - Answer: about 1 micrometer on 100x



size of bacillus cell - Answer: about 4 micrometers on 100x



size of spiral cell - Answer: about 15 micrometers on 100x



size of average bacteria - Answer: 0.2 micrometers to 3.0 micrometers



can reach a length of 10 micrometers



arrangements of bacilli - Answer: singles, diplo, strepto, palisades



arrangements of cocci - Answer: singles, diplo, strepto, tetrads, sarcinae, staphylo



arrangements of spiral - Answer: vibrio, spirilla, spirochete (axial filament), stella, cubic, coccobacillus

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