What is the process of contamination control? - Aseptic technique
What are pipettes - calibrated glass tubes used to measure and dispense small volumes of liquids
What color is the bigger vs. smaller micropipettors pipettes? - Green - bigger
Blue - smaller
What is the purpose of isolating...
MCB 2610 Lab Practical Exam 2024
What is the process of contamination control? - Aseptic technique
What are pipettes - calibrated glass tubes used to measure and dispense small
volumes of liquids
What color is the bigger vs. smaller micropipettors pipettes? - Green - bigger
Blue - smaller
What is the purpose of isolating bacteria? - To establish a PURE CULTURE, to be
able to study to study the physiology and morphology of a particular bacterial type
What is the quickest and most economical method of bacterial isolation? - Streak
plate method
What must you use to view items under 100x? - immersion oil
What does parfocal mean? - It means that if you focus the specimen in one
objective, it'll be in approximate focus in all objectives
What is a simple stain? - Staining procedures that only use one stain
What is a direct stain? - A simple stain that colors the bacteria
What is a negative stain? - Simple stain that stains the background but leaves the
bacteria unstained
, What is the purpose of heat fixing? - 1. Denaturing bacterial enzymes to prevent
autolysis
2. Enhances the adherence of bacterial cells to microscope slide
In Gram stains, why can't Gram negative bacteria retain the crystal violet? -
Because Gram negative bacteria have an outer membrane and a thinner layer of
peptidoglycan, which are easily depolarized and can only retain the counterstain
(Safranin-pink)
What are the 3 most common stain? - Gram, capsule, and spore stain
What are stains made of? - Usually salts, a few acids or bases, charged ions.
The ion that is colored contains a chromophore group
What is it called if the chromophore is a positive ion (like methylene blue)? - Basic
stain
What is it called if the chromophore is a negative ion? - Acidic stain
What causes endospore formation? - It's a response to certain environmental
conditions such as depletion of nutrients, heating, drying, and other unfavorable
conditions.
Heat used during staining does not cause sporulationn
Procedure for gram stain - flood violet - 1 min
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