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UTA Microbiology 2460 Chapter 9 2024 Actual Questions with Verified Answers, 100% Guarantee Pass
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UTA Micro 2460 Chapter 9.pdf file:///C:/Users/HP/Desktop/NIW/UTA%20Micro%202460%20ChapUTA Micro 2460 Chapter 9
1. Binary Fission: Most common form of bacterial reproduction
2. Binary Fission
4Basic Steps: 1.Growth of cell size and increase in cell components 2.Replication of
DNA
3.Division of the cytoplasm (cytokinesis)
4.Septum formation and division of daughter cells
3. Z ring Assembly: •Cytokinesis is directed by FtsZ protein
• FtsZ assembles Z ring to form divisome
• Divisome activates production of peptidoglycan and septum
4. GenerationTime: •Generation Time (Doubling Time) - time takes to double pop-
ulation
5. Generation Time
among species: •E. coli = 20 min. S. aureus = 30 min.
B. subtilis = 120 min.M. tuberculosis = 15-20 hrs
6. Calculating Population Size: •Growth is exponential (if resources are no con-
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,UTA Micro 2460 Chapter 9.pdf file:///C:/Users/HP/Desktop/NIW/UTA%20Micro%202460%20Chap
cern)
• Population can be predicted from any starting size
Nn= N02n
Nn - number of cells at generation n n
- number of generations
N0 - initial number of cells
•
• Number of generation may need to be calculated
• Ex. Generation time of 30 min = 48 generations in 24 hours
1 initial cell would result in more than 281 trillion cells after 1 day!
7. Growth Curve: •Closed cultures have finite resources (i.e. nutrients)
8. Culture density: the number of cells per unit volume
9. growth curve phases: lag phase, log phase, stationary phase, death phase
10. 1.Lag phase: - inoculum cells added and adjust to culture medium; no change in
population
11. 2.Log: (exponential) phase - binary fission occurs;cell replication > cell death
12. 3.Stationary phase: resources become depleted cell replication = cell death
13. 4.Death phase: 1.Death phase - endospores can form cell replication < cell
death
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,UTA Micro 2460 Chapter 9.pdf file:///C:/Users/HP/Desktop/NIW/UTA%20Micro%202460%20Chap
14. Lag Phase: •Initial cell numbers do not change
• Cells grow larger; metabolically active
• Damaged or shocked cells undergo repair
• Duration of the lag phase determined by many factors including:
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