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UTA Microbiology Exam 3 2024 Actual Questions with Verified Answers, 100% Guarantee Pass
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UTA Microbiology Exam 3.pdf file:///C:/Users/HP/Desktop/NIW/UTA%20Microbiology%20Exam%UTA Microbiology Exam 3
1. 4 Basic Steps of binary fission
ANS 1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
2. Z Ring Assembly
ANS FtsZ assembles Z ring to form divisome
3. Generation Time
ANS time it takes to double the population
4. Lag Phase
ANS Cells adjust to culture medium; no change in population
5. What factors determine the duration of the lag phase?
ANS Genetic makeup, media composition, initial inoculum size
6. During which phase of the growth curve is it most appropriate for the use of
antibiotics?
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ANS Log phase
7. Following the death phase of the growth curve, cells are considered the surviving
cells & are considered clinically important due to their association with chronic
infections, such as Tuberculosis.
ANS Persisters
8. Log Phase
ANS Binary fission occurs; cell replication > cell death
9. Stationary Phase
ANS Resources become depleted; cell respiration=cell death
10. Death Phase
ANS Endospores can form; cell replication < cell death
11. Close System Cultures
ANS Have finite resources
12. Open System Cultures
ANS -Nutrients and air are replenished
-Dead cells and waste are removed
-Beneficial for industrial microbiology
13. Microscopic Cell Count
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ANS Cells are counted under a microscope using a laser etched slide
14. In using the direct microscopic cell count method, you will be able to distinguish
between live vs dead cells.
ANS False
15. Fluorescence Staining
ANS Cells are counted under a microscope or flow cytome- ter
16. In fluorescent staining, the end product results in live cells staining
?
ANS Green
17. In fluorescent staining, the end product results in dead cells staining
?
ANS Red
18. Coulter counting
ANS Detects electrical resistance change due to cell density
19. Viable Cell Counts
ANS Samples are diluted and grown on solid media
20. What type of cells are counted when using the viable plate count method?-
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ANS Living
21. Countable Range
ANS 30-300 CFU/ml
22. Optical Density
ANS -Measured with spectrophotometer
-Light is passed thru culture and measured on other side
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