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AAB MT Molecular Diagnostics Enzymes Questions and Answers 2024
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AAB MT Molecular Diagnostics Enzymes
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AAB MT Molecular Diagnostics EnzymesEcoR1, HindIII, BamHI, NotI, and PacI are all examples of which class of restriction
enzymes? - answerClass II
This class of restriction enzymes account for at least 90% of enzymes used in molecular
biology? - answerClass IIP (Palindromic specificity)
How many base pairs are normally recognized by Class II restriction enzymes? -
answer4-8 base pairs (6-8 allows for larger fragments)
This restriction enzyme class typically recognizes modified (methylated) DNA? -
answerClass IV
This enzyme degrades ssDNA from either the 5' or 3' end? - answerExonuclease VII
This enzyme is one of the few enzymes that has 5' exonuclease activity? -
answerExonuclease VII
What is the enzyme that cleaves the extra nucleotides from the 5' end of a tRNA
precursor. Most forms of bacterial RNase P have a catalytic RNA subunit? -
answerRNase P (Ribonuclease P)
What is the enzyme that synthesizes DNA by linking together deoxynucleoside
monophosphates (dNMPs) in the order dictated by the complementary sequence of
nucleotides in a template DNA strand? - answerDNA polymerase
What is the enzyme that cleaves other proteins? - answerProtease
What is the enzyme that degrades RNA? - answerRNase (Ribonuclease)
What is the enzyme that degrades DNA by catalyzing the hydrolytic cleavage of
phosphodiester linkage in the DNA backbone? - answerDNase (Deoxyribonuclease)
This enzyme is used to unpack an organism's gene by separating two annealed
strands? - answerHelicase
This enzyme unwinds double standard DNA during replication? - answerHelicase
The RNA polymerase is activated by DNA helicase and prepares DNA for replication? -
answerDNA Primase
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