When does RNA-mediated gene silencing occur? correct answers After transcription of an mRNA but before translation
What is gene silencing? correct answers When RNA interferes and prevents protein synthesis through base pairing between ncRNA and mRNA.
What is NOT part of translational terminat...
BIOC 385-Exam 4 || All Answers Are Correct 100%.
When does RNA-mediated gene silencing occur? correct answers After transcription of an
mRNA but before translation
What is gene silencing? correct answers When RNA interferes and prevents protein synthesis
through base pairing between ncRNA and mRNA.
What is NOT part of translational termination? correct answers Uncharged tRNA recognition of
stop codon in the mRNA
Which type of interaction is NOT a functional mechanism by which IncRNA regulates gene
expression? correct answers IncRNA can be translated to produce a ribonucleoprotein complexes
that regulate transcription
Can: form base pairs with a region of ssDNA and alter transcription of the associated region
-form secondary structures that provide an assemb;y platform for factors that regulate gene
exression
-form basepairs with an mRNA and prevent translation
What is the term that dexcribes where DNA replication commences? correct answers Origin
How many origins should exist within a single replication bubble? correct answers 1
Within a replication bubble, how many replication forks are expected if bidirectional replication
is taking place? correct answers 2
Where do N-linked glycosylation proteins go to for further processing? correct answers In the
Golgi apparatus, they are transported between the ER and the Golgi via COPI and COPII
vesicles.
What is the role of omega in prokaryotic transcription? correct answers to ensure transcription
begins in the correct location
Which mechanisms are similar in prokaryotic transcription of mRNA? correct answers -RNA
synthesis
-Recruitment of RNA polymerase to DNA
Which of the following statements regarding the transcription and mRNA processign in
eukaryotes is NOT correct? correct answers NOT: transcription factors (TFIIA, TFIIB, TFIIE,
TFIIH) assemble on RNA polyerase II after it binds to TFIID/TBP.
Posttranscriptional mRNA decay can occur from both the 5' and 3' ends. WHat primary process
is common to both of these decay pathways? correct answers Deadenylation
, Which of the following properties are associated with the origin of replication in bacterial DNA?
correct answers three 13-bp sequences followed by four 9-bp sequences, both of which are high
in A-T content,, b/c A-T only have 2 hydrogen bonds
Which DNA polymerase is responsible for most DNA replication in E.coli? correct answers
DNA pol III
What direction is DNA polymerized? correct answers Always in the 5' to 3' direction
Which strand in DNA replication always synthesized continuously? correct answers The leading
strand
What increases processivity of DNA pol? correct answers The beta clamp
What causes supercoils to develop? what alleviates the supercoils? correct answers Helicase;
gyrase
Where in an anticodon are you likely to find inosine? correct answers 5';
the 5' nucleotide of the anticodon interacts with the 3' nucelotide of the codon, which is the
wobble position. Inosine can bind to adenine, cytosine, and uracil, allowing one anticodon to
bind to three codons.
What is a way that IncRNA could regulate gene expression? correct answers IncRNA would be
complement of the mRNA sequence transcribed from protein-coding gene.
Which transcription factor is NOT used to induce a pluripotent state? correct answers Oct4,
Sox2, and Nanog
Inducing: Oct4, Sox2, Klf4, c-Myc
Why would antibiotics that affect the ribosome kill bacteria without harming their host? correct
answers Prokaryotic ribosomes have different structure than eukaryotic ribosomes, so antibiotics
that bind to one cannot bind to another (70s vs. 80s)
What do each of these lac operons encode for:lacA, lacY, lacZ, lacL correct answers LacA:
produces lac transacetylase
LacY: lac permease
LacZ: B-galactosidase
LacL: separate and produces the lac repressor
What is the role of mRNA 5' cap in eukaryotic translation? correct answers It forms a complex
with the poly(A) tai, which orients the ribosome on the mRNA
When is the 5' m7G cap added to an mRNA transcript? correct answers Added by guanine-N7
methyltransferase when the transcript is 25-30 nucleotides in length, which occurs early in the
elongation process
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