what is the size of prokaryote vs eukaryote ribosome
Prokaryote is 70S
Eukaryote is 80S
what is the additional RNA in the eukaryotic ribosome
5.8S RNA
How many proteins in the eukaryote ribosome
about 80
usually located around the outside of the subunit
what is the special thing about the ribosome
only natural ribozyme that does more than shuffle phosphodiester bonds in nucleic
acids
, what is the shape of a TRNA
clover leaf shape
what is at the bottom of a TRNA structure
anticodon loop
what does the anti-codon loop do
it is what pairs the codon in the messenger RNA, creating a 3 base pair interaction
What is located at the 3' end of the tRNA (at the top)
located at the acceptor stem- CCA
Stands for A76- where amino acid gets joined on.
What happens in amino acid activation
amino acid and ATP bind catalytic site.
Nucleophilic attack by aa-amp
what is the second step of the amino acid reaction
hydroxyl group of adenine 76 of tRNA attacks the carbonyl carbon of the adenylate
= forms aminoacyl-tRNA and AMP
what catalyses the 2 step amino acid reaction
Aminoacyl-tRNA synthetases
whats the key step of peptide bond formation in translation
to move the ribosome along the message by 3 bases to bring the next codon into the
next site
what drives translation elongation
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