UTA Microbiology Exam 3 Questions With Correct Answers
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Course
UTA Microbiology
Institution
UTA Microbiology
UTA Microbiology Exam 3 Questions
With Correct Answers
4 Basic Steps of binary fission - answer1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly - answerFtsZ assembles Z ring to form divisome
Generation Time - answertime...
BRIGHTSTARS EXAM STUDY SOLUTIONS 8/15/2024 2:05 PM
UTA Microbiology Exam 3 Questions
With Correct Answers
4 Basic Steps of binary fission - answer✔✔1. Growth of cell size
2. Replication of DNA
3. Division of cytoplasm
4. Septum formation & cell division
Z Ring Assembly - answer✔✔FtsZ assembles Z ring to form divisome
Generation Time - answer✔✔time it takes to double the population
Lag Phase - answer✔✔Cells adjust to culture medium; no change in population
What factors determine the duration of the lag phase? - answer✔✔Genetic makeup, media
composition, initial inoculum size
During which phase of the growth curve is it most appropriate for the use of antibiotics? -
answer✔✔Log phase
Following the death phase of the growth curve, ____ cells are considered the surviving cells &
are considered clinically important due to their association with chronic infections, such as
Tuberculosis. - answer✔✔Persisters
Stationary Phase - answer✔✔Resources become depleted; cell respiration=cell death
Death Phase - answer✔✔Endospores can form; cell replication < cell death
Close System Cultures - answer✔✔Have finite resources
Open System Cultures - answer✔✔-Nutrients and air are replenished
-Dead cells and waste are removed
, BRIGHTSTARS EXAM STUDY SOLUTIONS 8/15/2024 2:05 PM
-Beneficial for industrial microbiology
Microscopic Cell Count - answer✔✔Cells are counted under a microscope using a laser etched
slide
In using the direct microscopic cell count method, you will be able to distinguish between live vs
dead cells. - answer✔✔False
Fluorescence Staining - answer✔✔Cells are counted under a microscope or flow cytometer
In fluorescent staining, the end product results in live cells staining ___________? -
answer✔✔Green
In fluorescent staining, the end product results in dead cells staining ___________? -
answer✔✔Red
Coulter counting - answer✔✔Detects electrical resistance change due to cell density
Viable Cell Counts - answer✔✔Samples are diluted and grown on solid media
What type of cells are counted when using the viable plate count method? - answer✔✔Living
Countable Range - answer✔✔30-300 CFU/ml
Optical Density - answer✔✔-Measured with spectrophotometer
-Light is passed thru culture and measured on other side
Which method of measuring growth is considered an indirect approach? - answer✔✔Optical
density
Which method of measuring growth can be used on living or dead cells? - answer✔✔Fluorescent
stain
How is the pour plate technique set up? - answer✔✔The sample is mixed with a warm agar
ranging from 45-50 C, poured onto sterile plated and swirled to mix
How is the spread plate technique set up? - answer✔✔The sample is poured onto a solid medium
and spread using a L spreader
What cell counting method is used for counting colonies from drinking water? -
answer✔✔Membrane filtration
What method of measuring growth is used in food/water testing? - answer✔✔MPN (most
probable number)
Steps of Biofilm Formation - answer✔✔1. Attachment of planktonic cells
2. Cells become sessile
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