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PHLEBOTOMY ORDER OF DRAW STUDY NHCO EXAM QUESTIONS AND ANSWERS
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PHLEBOTOMY ORDER OF DRAW STUDY NHCO EXAM QUESTIONS AND ANSWERS
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PHLEBOTOMY ORDER OF DRAWSTUDY NHCO EXAM QUESTIONS AND
ANSWERS
Lab responsible for blood cultures? - Answer-Microbiology
Additive in blood cultures? - Answer-Sodium polyanethole sulfonate- SPS (Nutrient
Broth)
How and what do you clean for blood culture? - Answer-ChloraPrep (not alc) at injection
site and wipe down the tops of collection bottles
What order do you fill blood culture bottles? - Answer-Aerobic first and then anaerobic
(butterfly tubing contains air, hence why aerobic is first)
What does STAT mean? - Answer-Test must be carried out immediately
T/F) Blood cultures are usually ordered STAT or as timed specimens - Answer-True
What is the purpose of sodium polyanethole sulfonate (SPS)? - Answer-To promote
rapid growth of pathogenic/infectious microorganisms in a blood sample (for
identification)- blood culture bottles
What tubes come before and after blood cultures? - Answer-No tubes before, discard
tube after (clear or glass red top).
What needle should you use for a blood culture? - Answer-Use a winged infusion set
(butterfly) to prevent nutrient broth introduction to blood stream (may differ by employer)
Which red top tube (glass/plastic) do you use as a discard? - Answer-Glass (glass red
tops contain no additives)
What is a discard tube used for? - Answer-Used to prevent thrombin and other cells
from polluting the samples drawn in following tubes.
What tubes come before and after a discard tube? - Answer-Blood culture bottles
before, light blue top tube after
What tubes come before and after a light blue top? - Answer-Discard tube before (clear,
red glass), royal blue with red stripe after
T/F) the royal blue with red stripe tube has no additives - Answer-True
, Does the royal blue red stripe tube yield serum or plasma? - Answer-Serum
What is the difference between a glass red top tube (no additive) and a royal blue red
stripe tube? - Answer-The royal blue red stripe tube contains no nickel
What is the difference between a purple top tube and a royal blue lavender/blue stripe
tube? - Answer-The royal blue lavender/blue stripe tube contains no nickel
What 2 tests are royal blue tubes used for? (Red stripe or lavender/blue stripe) -
Answer-Lead (Pb) poisoning, Heavy metal toxicology
Lab responsible for royal blue tubes? (Red stripe or lavender/blue stripe) - Answer-
Toxicology
How many times should you invert royal blue tube? (Red stripe or lavender/blue stripe) -
Answer-8 times (but you will invert all tubes 8-10 times)
What tubes come before and after royal blue red stripe tube? - Answer-Light blue
before, plastic red top after
What tubes come before and after royal blue lavender/blue stripe tube? - Answer-
Lavender before, grey after
Lab responsible for light blue top tube? - Answer-Coagulation (or Hematology if a
smaller facility)
Additive in light blue tube? What does it do? - Answer-Sodium citrate- anticoagulant
(prevents blood from coagulating and using up coagulation factors before reaching the
lab)
How does sodium citrate work? - Answer-Sodium citrate prevents coagulation by
binding to the calcium in a specimen. This preserves the specimens coagulation factors.
How much should you fill the light blue tube top? And why? - Answer-All the way!
Sodium citrate requires a very specific blood to additive ratio (9 pets blood to 1 part
additive). There is a fill line on the tube
How much do you invert light blue tube? - Answer-3-4 times (but you will invert all
samples 8-10 times)
Does the light blue top tube yield serum or plasma? - Answer-Plasma
What must you do BEFORE collecting a light blue top tube? - Answer-You must draw a
discard tube. This prevents thromboplastin from entering the light blue tube.
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