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Chapter 5 Pathology of Body fluids Exam Test Guide Questions and Answers
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Describe how each of the following errors in reagent strip technique will affect test results: a. Leaving the reagent strip in the urine specimen while recording results of the previous specimen. - Answer-A reagent will leach from the strip, causing false negative results b. Failing to remove ...

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  • August 16, 2024
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Chapter 5 Pathology of Body fluids
Exam Test Guide Questions and
Answers
Describe how each of the following errors in reagent strip technique will affect test
results:
a. Leaving the reagent strip in the urine specimen while recording results of the previous
specimen. - Answer-A reagent will leach from the strip, causing false negative results

b. Failing to remove excess urine while withdrawing the strip from the specimen -
Answer-Runover among pads will invalidate results

c. Recording all results immediately after withdrawing the strip from the specimen. -
Answer-False negative results (particularly LE) because of insufficient reaction time.

d. Using a Chemstrip color chart with a Multistix reagent strip. - Answer-Inability to
correctly correlate reaction colors due to differences in reagent strips.

How will failure to allow a refrigerated specimen to warm to room temperature before
testing
affect reagent strip testing? Why? - Answer-Results will be falsely decreased; enzymatic
reactions are temperature-dependent.

How are reagent strips protected from deterioration caused by the following?
a. Moisture - Answer-desiccant in the container and tightly closing the container after
removing strips

How are reagent strips protected from deterioration caused by the following
Volatile chemicals - Answer-tightly closing containers and avoiding using strips in the
presence of fumes.

How are reagent strips protected from deterioration caused by the following
c. Light - Answer-opaque containers

How are reagent strips protected from deterioration caused by the following
d. Heat - Answer-storing at temperatures below 30 degrees Celsius.

What is the significance of the expiration date stamped on reagent strip containers? -
Answer-The last date the strips can be guaranteed to produce accurate results.

List four situations in which positive and negative controls must be run on reagent strips.
- Answer-At least once every 24 hours; a new bottle is opened; questionable results are
obtained; there is concern over reagent strip integrity.

, Explain the relationship of urine pH to the formation of crystals and renal calculi. -
Answer-Urine pH (acidic or alkaline) determines the type of crystals and calculi formed.

What is the significance of a urine pH of 9? - Answer-The specimen has remained
unpreserved at room temperature for too long ( a new specimen should be collected).

Why do reagent strip pH reactions use both methyl red and bromothymol blue
indicators? - Answer-The pH range needed is too broad for one indicator to measure.

Describe the unique solubility characteristics of Bence-Jones protein - Answer-Bence
jones protein precipitates at 40 degrees C to 60 degrees C (40-60) and dissolves at
100C.

Differentiate between glomerular and tubular proteinuria. - Answer-Glomerular
proteinuria occurs when protein (primarily albumin) passes through the glomerulus,
whereas tubular proteinuria represents defects in the tubular reabsorption of low
molecular weight proteins usually filtered and reabsorbed.

How does detection of microalbuminuria affect patient treatment? - Answer-The patient
requires better stabilization of blood glucose levels.

Briefly explain the principle of the protein error of indicators. How does a highly alkaline
urine affect this? - Answer-When the pH remains constant, certain indicators change
color in the presence of protein; highly alkaline urine will override the reagent strip pH
buffer, thus producing a color change related to pH, not protein.

State a pathologic reason that would cause a negative reagent strip protein reaction and
a
positive SSA test result. - Answer-Multiple Myeloma

Explain why glycosuria occurs in the presence of hyperglycemia. - Answer-Blood
glucose levels greater than the renal threshold for glucose produce glucose
concentrations in the filtrate that exceed the maximum reabsorptive capacity of the
tubules.

Explain why the following people would exhibit hyperglycemia and glucosuria:
a. A person with hyperthyroidism - Answer-The hormone thyroxine opposes the action
of insulin by breaking down glycogen to glucose.

Explain why the following people would exhibit hyperglycemia and glucosuria:
b. A person under extreme stress. - Answer-Epinephrine inhibits insulin secretion

How can glycosuria occur in the absence of hyperglycemia? - Answer-Renal tubular
dysfunction

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