Exam (elaborations)
Electrophoresis Exam – Questions With Solutions
- Course
- Institution
Electrophoresis Exam – Questions With Solutions
[Show more]
Content preview
Electrophoresis Exam – Questions With SolutionsElectrophoresis basic principle Right Ans - charged particles or molecules
when placed in an electrical field will migrate towards the opposite charge
Migration depends on Right Ans - charge, size and molecular weight, shape,
strength of electrical field, type of support media, pH and time
What is the largest effect on migration Right Ans - charge. Protein take on
charge depending on what soln. pH is = amphoteric.
Size and molecular weight Right Ans - smaller molecule migrate faster and
larger molecule migrate slower
shape Right Ans - protein denatures and will slow migration. round
(newer) go faster
strength of electrical feild Right Ans - increase voltage will increase
migration up to a point. If increase voltage too much then heat will denature
proteins and evaporate fluid which will increase conc. of buffer.
type of support media Right Ans - agar based is most common
pH Right Ans - Buffer must be the right pH to create a negative protein
charge
Time Right Ans - limit time because at some point all proteins will migrate
to the end.
Charge effect on migration: 2+ molecule will migrate 2X as far as Right Ans
- -2 charge
How many fractions for serum protein Right Ans - 5
We make protein negative (anion) so they migrate toward the Right Ans -
anode (positive)
, Bridge Right Ans - where the serum goes on the electrophoresis. Put
sample in strips with a pipette on the media.
Buffer moves up both sides by Right Ans - osmosis. Particles in buffer move
toward cathode.
Electrophoresis instrument Right Ans - power supply, support media,
buffer, bridge
After electrophoresis Right Ans - fix, stain and denseotometer to measure
Quantitate based on Right Ans - thickness of band and tell what it is based
on where it migrates
Parameters affecting electrophoresis Right Ans - physical properties of
proteins, buffer, electrical field, electro-osmosis, wick flow, support media
Proteins are amphoteric Right Ans - + and = charge
pH < pI (isoelectrical charge) Right Ans - positive charge
pH = pI (isoelectrical charge) Right Ans - no net charge. Isoelectric point
pH > pI (isoelectrical charge) Right Ans - negative charge
The further away pH is from pI Right Ans - the greater the net negative (or
positive charge)
Albumin pI Right Ans - 4.9
Gammaglobulins pI Right Ans - 7.4
In blood what charge do albumin and gammaglobulin have based on pI
Right Ans - blood pH = 7.35-7.45 --Albumin pH>pI therefore Albumin is
negative---Gammglobulin could be neutral, pos or neg. depending on exact pH
If we have a buffer with a pH of 8.6 what is charge of albumin and
gammaglobulin based on pI Right Ans - Albumin and gammaglobulin pH>
pI so both are negative. Albumin there is a greater difference so it will be more
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Zendaya. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67866 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now