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Gel Electrophoresis Lab – Complete Study Guide
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Gel Electrophoresis Lab – Complete Study Guide
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Gel Electrophoresis Lab – Complete Study GuideAgarose Gel Electrophoresis is? Right Ans - a widely used analytical
method that separates molecules on the basis of charge, size, shape
On what basis does electrophoresis separate molecules? Right Ans -
charge, size, shape
What are the three types of charged biomolecules that electrophoresis is
particularly useful in separating? Right Ans - DNA, RNA, proteins
What is gel made of? Right Ans - dissolving agarose powder
In what is the gel submerged? Right Ans - In a buffer-filled chamber which
contains electrodes
What substance is used to make samples denser? Right Ans - glycerol or
sucrose
Why do samples need to be more dense than the buffer? Right Ans - So that
they can be loaded with a micropipette into the well created in the gel by
comb
What is the purpose of the buffer? Right Ans - it completes an electric
circuit between the electrodes
Which way do negatively charged molecules migrate? Right Ans - towards
the positive electrode
What is the purpose of buffer salts? Right Ans - they serve to make water a
better conductor of electricity
Why is it important for the buffer to maintain pH? Right Ans - important
for the charge and stability of molecules
What characteristics of agarose makes it useful for electrophoresis? Right
Ans - microscopic pores which act as molecular sieves
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