Exam (elaborations)
Gel Electrophoresis Online Simulation – Questions With Accurate Solutions
- Course
- Institution
Gel Electrophoresis Online Simulation – Questions With Accurate Solutions
[Show more]
Content preview
Gel Electrophoresis Online Simulation – QuestionsWith Accurate Solutions
What is electrophoresis used for? Right Ans - Pushing DNA strands through
the gel filter (Moving DNA)
What is the material of the gel similar to? Right Ans - A sponge made of
Jello
Describe how the DNA fragments migrate through the gel matrix. Right Ans
- DNA strands of the same length will move at the same speed and group up
together, they move through holes in the sponge.
What moves farther - shorter or longer fragments? Why? Right Ans -
Shorter because they move quicker
If two lanes had bands/fragments at the same location could you assume that
the DNA at these locations was the same length? Why or why not? Right
Ans - Yes because those bands moved the same distance meaning the the
same length because different length fragments move different distances.
What ingredients/materials are necessary to perform an electrophoresis
experiment? Right Ans - Powdered agarose, buffer, a flask, a microwave,
the gel mold and the gel comb.
Why do you need to use buffer? Right Ans - A buffer is a salt water solution
that will let electrical charges flow through the gel.
Why do you need a microwave? Could you use something else? What?
Right Ans - To heat and melt the agarose and buffer together. yes, You could
also use a hot plate and hot plate stirrer.
What is the tape for on the gel casting tray? Right Ans - To hold the melted
agarose
What is the comb for? Right Ans - To create wells
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Zendaya. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $8.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67866 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now