Lab 2: Culture Media Preparation Questions and Answers Graded A+
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Course
RHFAC
Institution
RHFAC
Lab 2: Culture Media Preparation Questions and Answers Graded A+
Solutions that contain nutritional requirements for bacterial growth:
culture media
Culture media can be in what form?
liquid (broth), semisolid, or solid.
Solidifying agent derived from seaweed, which is a complex ca...
Lab 2: Culture Media Preparation
Questions and Answers Graded A+
Solutions that contain nutritional requirements for bacterial growth: - answer culture
media
Culture media can be in what form? - answer liquid (broth), semisolid, or solid.
Solidifying agent derived from seaweed, which is a complex carbohydrate with no
nutritional value. - answer Agar.
Agar concentration for solid: - answer 1.5 to 1.8%
Agar concentration for semisolid: - answer less than 1%.
Broth/liquid media is used to grow: disadvantage: - answer large amounts of
bacteria. Disadvantage: you cannot determine the different types of cells from a broth
culture because the bacteria will be in suspension.
Solid media allows for: - answer mixed cultures of bacteria to be isolated into
discrete colonies.
The discrete colonies on solid media represent: - answer multiplication of a single
cell and signifies a particular species.
Dragging a contaminated swab on agar will form: - answer different colonies.
Broth media is usually in not plates, but: - answer tubes or flasks.
Agar in test tubes are called: - answer agar deeps.
You can also slant test tubes, and create: - answer agar slants.
Agar is most commonly poured into sterile petri dishes to generate: - answer agar
plates.
Two types of media: - answer chemically defined and complex media.
Chemically defined media are used when: - answer an organism's exact nutritional
needs are known and its chemical makeup is known (i.e. glucose salt broth). The
precise nutritional requirements of an organism must be known.
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