ASCP CG Exam (Classic Cytogenetics) Latest
Update 2024-2025 450 Questions and 100%
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A good banding pattern will be expected when we observe chromosomes before
banding under the phase scope with the following appearance: - CORRECT ANSWER:
Dark.
A PB sample is clotted. What do you do? - CORRECT ANSWER: Break up the clot.
About how long does mitosis last? - CORRECT ANSWER: About 15mins.
Adding the drug Colcemid to cell cultures can cause all of the following phenomenons to
occur except: - CORRECT ANSWER: Cell swelling.
All of the following have buffering capability except: - CORRECT ANSWER: L-
Glutamine.
All of the following information is required during the specimen set up procedure except:
- CORRECT ANSWER: Infectious agent.
All of the following will result in culture failure except: - CORRECT ANSWER: Transport
at room temp.
At 24hrs of exposure to PHA, what will be happening to T-lymphocytes? - CORRECT
ANSWER: Increase in RNA synthesis, signaling a transformation of lymphocytes to
lymphoblasts.
At 48hrs of exposure to PHA, what will be happening to T-lymphocytes? - CORRECT
ANSWER: Most of the mitoses observed are the result of one cell division.
,At 72hrs of exposure to PHA, what will be happening to T-lymphocytes? - CORRECT
ANSWER: Most cells have undergone 2 cell divisions.
At 96hrs of exposure to PHA, what will be happening to T-lymphocytes? - CORRECT
ANSWER: Cells have exhausted media and begin to die.
Describe the physical differences between villi and decidua. - CORRECT ANSWER:
The villi will be branched in appearance and lighter as compared to the decidua, which
will be more sheet-like and darker.
Does positive or negative heterpyknoesis stain dark? - CORRECT ANSWER: Positive.
During slidemaking all of the following factors are important except for: - CORRECT
ANSWER: Slides are 1st being dipped in acetic acid.
For how long can cells be stored in -90 degrees C? - CORRECT ANSWER: For up to a
year.
For solid stains, how can we sequentially stain? - CORRECT ANSWER: Q-banding and
AgNOR; G-banding and FISH.
Hoe does the culture media appear when a contamination has occurred? - CORRECT
ANSWER: Cloudy and turbid.
How are CVS samples retrieved? - CORRECT ANSWER: Transcervically and
transabdominally.
How are percutaneous umbilical blood samples (PUBS) retrieved? - CORRECT
ANSWER: Transabdominally with the assistance of ultrasonography.
How are slides aged at RT? - CORRECT ANSWER: Airtight for 3 days to 6 weeks.
,How can we chemically age slides? - CORRECT ANSWER: Treat with 15% H2O2 (2-
7mins) prior to banding.
How do clastogens affect the DNA repair system in normal and abnormal cells? -
CORRECT ANSWER: Normal cells affected by clastogens will be able to repair most of
the damage caused, whereas the abnormal cells will not.
How do slides need to be stored? - CORRECT ANSWER: In an airtight container with
temp and humidity control.
How do we confirm a mycoplasma contamination? - CORRECT ANSWER: By staining
with DAPI and Hoechst 33258.
How do we dehydrate a slide? - CORRECT ANSWER: By exposing the slide to ethanol
mixtures in the opposite direction of hydration.
How do we denature DNA for FISH? - CORRECT ANSWER: We treat in 70%
formamide/2xSSC at 70 degrees C.
How do we enhance spreading? - CORRECT ANSWER: By dropping on wet slides and
dropping at a 45 degree angle from top to bottom.
How do we hybridize DNA in FISH? - CORRECT ANSWER: The probe is hybridized
onto slides at 37 degrees C for 30mins to 16hrs depending on probe used.
How do we hydrate/rehydrate a slide? - CORRECT ANSWER: By exposing it to various
dilutions of ethanol moving from a low percent of water to a higher percent of water;
100%-90%-80%-70%-EtOH.
How do we know if a sample is amniotic fluid or maternal urine? - CORRECT ANSWER:
Normal AF is straw colored and passes the Fern Test.
, How do we know that the slides are properly cleaned? - CORRECT ANSWER: They
retain, rather than repel, water when dipped.
How do we reduce cellular contamination of a chorionic villus sample? - CORRECT
ANSWER: By removing the adherent maternal decidua.
How do we stimulate the growth of neoplastic cells or B-cells neoplasms? - CORRECT
ANSWER: Run one unstimulated culture and one with a B-cell mitogen.
How do we store a bone marrow sample if it cannot be cultured within 4 hours? -
CORRECT ANSWER: Add to media and store at room temp.
How do we store fixed cell pellets? - CORRECT ANSWER: At -20 degrees C for 2-5yrs
in fixative.
How do we test for chromosome breakage syndromes? - CORRECT ANSWER: By
adding clastogens.
How do we troubleshoot slides drying too quickly, without adjusting the temperature or
humidity of the thermotron? - CORRECT ANSWER: Methanol can be added to the fix or
cold slides may be used.
How do we troubleshoot slides drying too slowly without adjusting the temperature or
humidity of the thermotron? - CORRECT ANSWER: Warm slides may be used or airflow
around the slides may be increased.
How do you avoid cellular contamination of prenatal specimens? - CORRECT
ANSWER: Discard the 1st 1-2 cc of amniotic fluid drawn.
How do you avoid cellular contamination of solid tumor specimens? - CORRECT
ANSWER: Dissect away normal parenchyma and fatty tissue.
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