Portage Microbiology Exam #5 Questions and
Answers (100% Pass)
Define the concept of universal precautions (short answer) - ✔️✔️Universal precautions means
any and all samples, whether known or unknown, are to be treated as potentially hazardous
(or pathogenic) materials
List at lease 3 observations a researcher would be sure to note while assessing and unknown
microbial sample (short answer) - ✔️✔️Need to note:
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
While observing an unknown sample of limited amounts, a researcher must determine the
following observations: 1) the presence of any motility and 2) its Gram status using the same
sample- the liquid sample cannot be divided. Which would you determine first and why?
(short answer) - ✔️✔️You must determine motility before determining Gram status. Motility
requires a wet mount, while gram staining requires heat fixing the sample. If one were to
begin with the gram stain, the heat fixation process would kill the organism, making any
observations regarding motility impossible. The correct approach would be to place the liquid
culture on a glass slide and determine its motility status. Next, the same liquid culture can be
heat fixed and gram stained.
A facultative anaerobe is a microorganism capable of growth under what conditions? (short
answer) - ✔️✔️A facultative anaerobe is capable of growth under aerobic (with oxygen) and
anaerobic (without oxygen) conditions
True or False: The Lancefield groups are used to subdivide antigenic groups of alpha-
hemolytic Streptococcus - ✔️✔️False. The Lancefield groups are used to subdivide beta-
hemolytic strep
The distinctions for Lancefield subgroups lie in its: (select all that apply)
A. Catalase activity
B. Carbohydrate composition of antigens
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C. Hemolytic activity
D. All of the above - ✔️✔️B. Carbohydrate composition of antigens
Rheumatic fever displays _____ hemolytic activity and occurs when _____ if left untreated -
✔️✔️Beta: strep. Pharyngitis
True or False: Under most circumstances, staphylococcus can be found in ~80% of human
population where it remains non-symptomatic - ✔️✔️False. Staphylococcus in only found
(non-symptomatic) in ~30% of the human population
Define commensal bacteria (short answer) (know term and definition first) - ✔️✔️The
bacterium neither harms nor benefits from the host from which it obtains nutrients
How can staphylococcus be differentially tested? (short answer) - ✔️✔️Staphylococcus species
are capable of growth in the presence of bile salts
Symptoms of folliculitis - ✔️✔️Pus-filled lesions on skin or hair
Symptoms of scalded-skin syndrome - ✔️✔️Ruptured-pustules; treated with penicilin
Symptoms of Impetigo - ✔️✔️Childhood skin disease near mouth/nose
Symptoms of conjuctivitis - ✔️✔️Infections of thin, transparent scieral tissue
Symptoms of ophthalmia neonatorum - ✔️✔️Infection occurs at time of birth
True or False: the causative agent of conjunctivitis can be either bacterial or viral - ✔️✔️True.
There are bacterial (staph) and viral forms of conjunctivitis, both resulting in the
inflammation of the conjunctiva and 'pink eye'
True or False: Tuberculosis is best identified by a gram stain - ✔️✔️False. Mycobacterium
show poor Gram staining and show be screened via an acid-fast stain
True or False: You can become infected with TB simply by sharing a drink with someone
who is currently infected with TB - ✔️✔️False. As per the CDC guidelines, simply shaking
someone's hand, sharing a drink, or even kissing someone cannot spread TB
Identify the disease based on the following (select all that apply): It is a small, acid-fast rod
usually remaining asymptomatic for up to 20 years. It affects the skin, nerves, upper
respiratory tract and eyes of infected individuals
A. Tuberculosis
B. Leprosy
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