BIOD 171 PORTAGE LEARNING EXAM 3 2024/2025
COMPLETE QUESTIONS WITH VERIFIED
ANSWERS | RATED A+
Define the measurements micrometer and nanometer. - Answer>>>A micrometer is onemillionth
of a meter and a nanometer is one-billionth of a meter.
What are the two critical factors that influ...
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, Define the measurements micrometer and nanometer. - Answer>>>A micrometer is one-
millionth of a meter and a nanometer is one-billionth of a meter.
What are the two critical factors that influence your ability to see an object? - Answer>>>The
resolution and the contrast. Resolution is the distance between two objects where you can still
see them as separate. Contrast is the difference in light between two objects.
If you wish to increase the amount of light going into a microscope, what part would you adjust?
- Answer>>>The iris diaphragm.
As light passes through a microscope, what is the last piece that light passes before reaching your
eyes? - Answer>>>Once light passes through the sample and the objective lens, it passes through
the OCULAR LENS.
How is the total magnification of an object calculated? - Answer>>>It is calculated by
multiplying the power of the objective and the power of the eyepiece.
What is one limitation of fixing your sample? - Answer>>>Fixing your sample requires you to
irreversibly kill your sample.
This can make it impossible to determine the cell movement. You could distort the shape and
structure.
Phase-contrast microscopy provides what benefits to imaging? - Answer>>>Phase-contrast
microscopy provides detailed images without staining.
What is the distinguishing feature of dark field microscopy? - Answer>>>Dark field microscopy
reflects light off of the specimen at an angle..
Unlike brightfieqld microscope, fluorescence microscopes illuminate samples through what
spectrum? - Answer>>>The Ultraviolet Spectrum.
What is the primary difference between TEM and SEM? - Answer>>>The electron passes
through the sample during TEM where as the electron is reflected off the sample during SEM.
Gram staining is based on what basic principle? - Answer>>>With the basic observation that
different types of bacteria react differently to various dyes.
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