overview of tissue preparation - CORRECT ANSWER 1) fixation
2) dehydration
3) clearing
4) embedding
5) sectioning
6) mounting
7) staining
light or electron - CORRECT ANSWER the ultimate goal of sample preparation is to enable the transmission of a _____ beam through the tissue (sample) t...
overview of tissue preparation - CORRECT ANSWER 1) fixation
2) dehydration
3) clearing
4) embedding
5) sectioning
6) mounting
7) staining
light or electron - CORRECT ANSWER the ultimate goal of sample preparation is to enable the
transmission of a _____ beam through the tissue (sample) to produce a visible image
requirements of sample preparation - CORRECT ANSWER - preparing the tissue for examination
- slicing of the tissue into thin sections
- attaching the tissue to either a glass slide (LM) or to a wire grid (TEM)
fixation - CORRECT ANSWER step of tissue preparation that aims to avoid autolysis or degradation of the
tissue by bacteria and to preserve the structure and molecular composition of the tissue
chemical solutions that stabilize the tissue - CORRECT ANSWER requirements for fixation --> LM uses
formaldehyde and TEM uses glutaraldehyde followed by osmium tetroxide
dehydration - CORRECT ANSWER step of tissue processing that aims to extract water from fixed tissue
fragments by successively bathing them in a graded series of ethanol and water mixtures, ultimately to
pure ethanol
, clearing - CORRECT ANSWER step of tissue processing that aims to make the tissue sample transparent
by replacing the ethanol with a solvent miscible with the embedding medium (like xylene)
embedding - CORRECT ANSWER step of tissue processing that aims to embed (enclose) the tissue in a
hard mold for easy cutting --> can use either paraffin wax or epoxy resin
sectioning - CORRECT ANSWER step of tissue processing that aims to slice the embedded tissue using
either a microtome (for LM) or an ultramicrotome (for TEM)
formaldehyde - CORRECT ANSWER chemical solution that is used to stabilize tissues for light microscopes
(LM) --> reacts with amine groups
amine groups - CORRECT ANSWER formaldehyde (used for LM) reacts with ______ in tissue samples
glutaraldehyde and osmium tetroxide - CORRECT ANSWER chemical solutions used to stabilize tissues for
transmission electron microscopes (TEM)
preserves membranes - CORRECT ANSWER a buffered solution of osmium tetroxide _____ in tissue
samples (for TEM)
graded ethanol and water solutions - CORRECT ANSWER requirements for dehydration step of tissue
preparation
series; water; ethanol - CORRECT ANSWER in dehydration, the tissue sample gets bathed in a ____ of
ethanol and water mixtures --> start out with a solution of pure ____, and progressively add ethanol to
the solution until it is pure ____
replacement of ethanol with xylene - CORRECT ANSWER requirement for the clearing step of tissue
preparation
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