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HTL ASCP EXAM- FIXATIVES (N) QUESTIONS AND ANSWERS WITH SOLUTIONS 2024 $14.99   Add to cart

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HTL ASCP EXAM- FIXATIVES (N) QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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HTL ASCP EXAM- FIXATIVES (N) QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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  • August 11, 2024
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  • 2024/2025
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HTL ASCP EXAM- FIXATIVES (N)
QUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate
and preserve the nucleoproteins and fix the nuclei. - ANSWER Acetic Acid



Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as
additive to counter-act the shrinking effect of other fixatives. - ANSWER Acetic Acid



Non coagulant and additive fixative that reacts with amino groups that link protein chains together. -
ANSWER Formaldehyde



Greatest binding occurs between pH 7.5-8. - ANSWER Formaldehyde



Preserves lipids but does not make them insoluble and can be lost during subsequent processing. -
ANSWER Formaldehyde



Does not preserve carbohydrates but stabilizes and fixes the proteins to trap glycogen. - ANSWER
Formaldehyde



Penetrates tissue quickly, fixes slowly and has the least shrinking effect any fixative. - ANSWER
Formaldehyde



Beside ethanol and acetone, this hardens tissue more than any other fixative. - ANSWER Formaldehyde



Formed when acidic (<6.0 pH) solutions of formaldehyde act on bloody tissue. - ANSWER
Microcrystalline dark brown pigment



When formalin pH drops below 6.0 this pigment forms and can be removed by alcoholic picric acid or
alkaline alcohol. - ANSWER Black acid hematin / formalin pigment

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