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ASCP CG CERTIFICATION PRACTICE QUESTIONS AND ANSWERS WITH SOLUTIONS 2024 $14.99   Add to cart

Exam (elaborations)

ASCP CG CERTIFICATION PRACTICE QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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  • ASCP CERTIFICATION
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  • ASCP CERTIFICATION

ASCP CG CERTIFICATION PRACTICE QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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  • August 11, 2024
  • 20
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ASCP CERTIFICATION
  • ASCP CERTIFICATION
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ASCP CG CERTIFICATION PRACTICE
QUESTIONS AND ANSWERS WITH
SOLUTIONS 2024
What is the best agent to use against mycoplasma? - ANSWER BM cyclin - a combination of two
antibiotics (tiamulin and minocycline) that effecticely targets mycoplasma growth



For successful harvest, each new batch of hypotonic solution should be evaluated by: - ANSWER
comparing the new lot to a lot already in use



Colagenase, pronase, and trypsin are: - ANSWER Proteolytic enzymes - break down tissues into single-
cell suspensions



When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation
with: - ANSWER Colcemid - mitotic spindle poison that halts cells at metaphase



Samples that arrive at the cytogenetics lab should first be: - ANSWER Logged in to the system



Phytohemagglutinin (PHA) stimulates which type of cells into division? - ANSWER T-cell lymphocytes



To avoid culture failure, monitoring reagents is crucial. When logging a culture failure, what is the MOST
important to document? - ANSWER Expiration dates of reagents used



Which type of microscope is used when cleaning a CVS (chorionic villus) sample? - ANSWER Dissection



Which cell type is most problematic if found in a CVS (chorionic villus) sample? - ANSWER Decidua
(maternal tissue)



When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the
mets, what is the most likely cause? - ANSWER innappropriate slide drying time

,What will help prevent microbial contamination and cross contamination of cultures? - ANSWER Asceptic
technique



When there are not enough mets to complete the study of a patient/case, what needs to be done next? -
ANSWER prepare additional slides from any remaining sample



When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what
could help fix the issue? - ANSWER Increading the drying time by using warm slides



What is true of an inverted microscope? - ANSWER the objectives are beneath the stage



High-resolution banding is indicated for a possible diagnosis of: - ANSWER DiGenorge syndrome --
microdeletion of 22q11, also called velocardiofacial syndrome



"velo" - soft palette,

"cardio" - congenital heart defects,

"facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread
eyes, etc



When verifying if a specimen is appropriate for cytogenetic analysis, what is the MOST important data to
accompany the specimen? - ANSWER Reason for referral



Which type of specimen should be requested for a cytogenetic analysis for turner syndrome? - ANSWER
Heparinized peripheral blood stored at room temperature



(NOT FROZEN)



A blood sample can be used to study the cytogenetics of a malignancy only if: - ANSWER Dividing
leukemic cells are present in circulation



All of the cultures in a CO2 incubator failed, what aspects of the incubator are important to monitor to
assess the source of the problem? - ANSWER Temperature,

, CO2 level, and

Humidity level



The most important factor affecting the quality of chromosomes viewed under a phase contrast scope is:
- ANSWER Slide drying after the cells have been dropped



What is the modal number (default number of chromosomes in a cell) for a Down syndrome
translocation carrier? - ANSWER 45



What is the best tissue to use for both a good success rate and abnormality detection in spontaneous
abortion miscarriages? - ANSWER Chorionic villi



What is a possible cause for a drying oven to not maintain a proper temperature? - ANSWER The door
does not seal properly



If a tech with a cold and sneezing does not take proper precautions while setting up amniotic fluid
samples, the specimen could become contaminated with: - ANSWER Mycoplasma



--> the most common contaminant by a sick tech and can be detected through a specific stain used on
the sample



mycoplasma can induce abnormalities in a sample, so it is important to avoid contamination



What about the metaphases on a slide are affected by slide making? - ANSWER Cell density - thru
dilution of the sample with fix



Banding - based on how dark/pale/refractive they are affects trypsin time



Chromosome spreading - affected by drying time



What test(s) should be ordered on a newborn with suspected Prader-Willi syndrome? - ANSWER High
resolution banding and FISH for the Prader-Willi locus on 15q11.2

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