100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
ASCP CG EXAM (CLASSIC CYTOGENETICS) QUESTIONS AND ANSWERS WITH SOLUTIONS 2024 $13.99   Add to cart

Exam (elaborations)

ASCP CG EXAM (CLASSIC CYTOGENETICS) QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

 9 views  0 purchase
  • Course
  • ASCP CERTIFICATION
  • Institution
  • ASCP CERTIFICATION

ASCP CG EXAM (CLASSIC CYTOGENETICS) QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

Preview 4 out of 35  pages

  • August 11, 2024
  • 35
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ASCP CERTIFICATION
  • ASCP CERTIFICATION
avatar-seller
Performance
ASCP CG EXAM (CLASSIC
CYTOGENETICS) QUESTIONS AND
ANSWERS WITH SOLUTIONS 2024
What are the basics of in situ hybridization? - ANSWER Pretreatment of slides with acid, extraction of
DNA with barium hydroxide, and incubation in 2xSSC to promote denaturation.



The following are indicators for amniocentesis except: - ANSWER A family history of an autosomal
recessive disorder.



When a bone marrow sample cannot be delivered to the lab within 4 hrs, the sample should: - ANSWER
Be put in media and kept at room temp.



A PB sample is clotted. What do you do? - ANSWER Break up the clot.



All of the following information is required during the specimen set up procedure except: - ANSWER
Infectious agent.



When should samples be logged in? - ANSWER As soon as they arrive in the laboratory.



How long do you wait before requesting an amniotic fluid if no cell attachment is observed? - ANSWER
10 days.



All of the following will result in culture failure except: - ANSWER Transport at room temp.



Which cells would you want to use for long term serial cytogenetic studies? - ANSWER Fibroblasts.



The following enzymes can be used to dissociate chorionic villus samples except for: - ANSWER RNAse.



Which of the following is not a necessary ingredient which is added to modified medium? - ANSWER
Antibiotics.

,All of the following have buffering capability except: - ANSWER L-Glutamine.



What type of media is necessary for the culture of Fragile X cases? - ANSWER Folic acid deficient media.



The most important reason why Biological Safety Caninets are used instead of Horizontal Laminar Flow
Hoods in the handling of human tissues is: - ANSWER To protect the technologist who is processing the
specimen.



In which of the following situations would one suspect bacterial contamination? - ANSWER The culture
media is turbid in appearance.



Which of the following can be used for serial culture from amniotic fluid? - ANSWER Fibroblasts.



Which of the following chemicals is used for cryopreservation? - ANSWER Dimethyl sulfoxide (DMSO).



Adding the drug Colcemid to cell cultures can cause all of the following phenomenons to occur except: -
ANSWER Cell swelling.



During slidemaking all of the following factors are important except for: - ANSWER Slides are 1st being
dipped in acetic acid.



A good banding pattern will be expected when we observe chromosomes before banding under the
phase scope with the following appearance: - ANSWER Dark.



Which of the following factors are not important for chromosome spreading? - ANSWER Mitotic index.



The best method to process slides for G-banding on the same day that they are dropped is: - ANSWER
Incubate the slide in 90°C for 1-3 hrs before banding.

,You are analyzing a clinical case and discover a small extra marker with satellites. What staining
technique would be most useful in determining the chromosomal origin of this marker? - ANSWER
DAPI/DA.



When using a fluorescein labeled probe during the FISH procedure, the appropriate counter stain used
should be: - ANSWER Propidium iodide.



When doing sequential staining which of the following is used to remove oil from slides? - ANSWER
Xylene.



What is the best banding technique to distinguish between 47,XY,+18 and 47,XYY? - ANSWER QFQ.



When the identification of paired chromosomes is the primary goal, which if the following banding
techniques should be used? - ANSWER G-banding.



When the addition of non homologous material to the q-arm of chromosome 16 is suspected after G-
banding, which if the following banding techniques should be used? - ANSWER C-banding.



Which chromosome is most different between C- and G-banding? - ANSWER 9.



What is not a criteria for G-band identification? - ANSWER Location of the telomere.



What do quinacrine and acridine orange dyes stain? - ANSWER AT rich DNA.



What type of container should be used for collecting peripheral blood and bone marrow specimens? -
ANSWER Green top tube coated with sodium heparin.



What type of container should be used for collecting solid tissue samples? - ANSWER A sterile container
containing media, supplemented with antibiotics.



How do you avoid microbial contamination? - ANSWER By using aseptic technique, sterile containers and
antibiotics/fungicides.

, How do you avoid cellular contamination of prenatal specimens? - ANSWER Discard the 1st 1-2 cc of
amniotic fluid drawn.



How do you avoid cellular contamination of solid tumor specimens? - ANSWER Dissect away normal
parenchyma and fatty tissue.



How much specimen is required for the successful analysis of a peripheral blood specimen? - ANSWER 5-
10 ml for adults and 3-5 ml for children.



How much specimen is required for the successful analysis of a bone marrow specimen? - ANSWER 1-3
ml.



How much specimen is required for the successful analysis of a prenatal specimen? - ANSWER 15-30 ml
of amniotic fluid collected in two separate containers.



How much specimen is required for the successful analysis of solid tissues? - ANSWER 5-25 mg of CVS, 1
mm^3 of skin biopsy and 1cm^3 of solid tumor.



What should the label of each collection container always have? - ANSWER Patient name and
identification number.



What does penicillin/streptomycin treat? - ANSWER Bacterial contamination.



When do you use a kanamycin/gentamycin combination instead of other antibiotics? - ANSWER When
an organism is resistant to penicillin/streptomycin. This combination is more broad-acting, but also a
more toxic antibiotic.



What do Amphotericin B, Fungizone and Mycostatin (Nystatin) treat? - ANSWER Fungal contamination.



True/False: Specimens should be shipped in direct contact with ice or dry ice. - ANSWER False.

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Performance. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $13.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

82215 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$13.99
  • (0)
  Add to cart