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(MB) ASCP STUDY EXAM QUESTIONS AND ANSWERS WITH SOLUTIONS 2024 $11.49   Add to cart

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(MB) ASCP STUDY EXAM QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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  • ASCP CERTIFICATION
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(MB) ASCP STUDY EXAM QUESTIONS AND ANSWERS WITH SOLUTIONS 2024

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  • August 11, 2024
  • 9
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ASCP CERTIFICATION
  • ASCP CERTIFICATION
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(MB) ASCP STUDY EXAM QUESTIONS
AND ANSWERS WITH SOLUTIONS 2024
What is metagenome? - ANSWER Sampling the genome sequence of a community of organisms.



Polymorphism involved for Irinotecan? - ANSWER UGT1A1



NGS related to deletions and insertions, coverage, depth? - ANSWER -Paired-end reads for detection of
indels

-Coverage is how much of your target has been read

-Depth is how many times a unique position has been covered



Polymerase for lagging strand? - ANSWER Euk - DNA Pol Alpha

Pro - DNA Pol I



Polymerase for leading strand? - ANSWER Euk - DNA Pol Delta and DNA Pol Epsilon

Pro - DNA Pol III



Polymerase used in LAMP? - ANSWER -Loop-mediated Isothermal Amplification utilizes Bst Polymerase

-Has helicase activity and activity at annealing/elongating temps



Highest resistance gene in enterococcus? - ANSWER vanA has the highest resistance in enterococcus



Resistance in mecA gene in MRSA codes for? - ANSWER mecA responsible for antibiotic resistance in S.
aureus (MRSA), and codes for PBP2a, which has low affinity for beta-lactam antibiotics like penicillin.



Important in gene expression, defense in eukaryotes? - ANSWER Person guessed mRNA - gene-
expression and codes for proteins that bind to DNA to promote/inhibit expression.



Donor/Recipient - what's informative loci? - ANSWER No matching alleles is informative, you can tell D
from R; non-informative would be both D&R matching.

, Bond between DNA and histones? - ANSWER Ionic - DNA is negative and histones are positive



Amino Acids that make up histones? - ANSWER Arginine and Lysine - positively charged amino acids



Multiplex modifications for low yield? - ANSWER Person guessed KCl.

Increasing Mg2+ could increase yield.

Decreasing stringency increases yield, but increases chances of mispriming



PCR troubleshooting - ANSWER Increase Temp --> Increase Stringency

Increase Probe Length --> Increase Stringency

Increase Probe Complexity --> Increase Stringency

Lower Salt Conc. --> Increase Stringency

Remove Mg2+ --> Increase Stringency

Less Time --> Increase Stringency

Add Formamide --> Lowers Stringency

Add Bovine Serum --> Lowers Stringency

Increase Probe Conc. --> Lowers Stringency



How does an amplification control help? - ANSWER Differentiates a true negative from a false negative

Amplification controls have a target that should always amplify, even if your sample does not amplify



Best molecular assay for Cystic Fibrosis? - ANSWER CF is caused by a 3b deletion: delF508

RFLP, PCR and Southern Blot are used.



What to use FISH for: - ANSWER PWS/AS can be detected with FISH

Gaucher - Sequencing or enzyme activity screening

FXS - RFLP, PCR, SSCP

TS - Sequencing or enzyme activity screening

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