Exam (elaborations)
BIOMG 3300 Unit 9 Objectives Questions with Correct answers.
- Course
- Institution
BIOMG 3300 Unit 9 Objectives Questions with Correct answers.
[Show more]
Content preview
BIOMG 3300 Unit 9 Objectives Questions withCorrect answers
5 procedures in cloning Correct Answer--obtaining DNA to be cloned
-selecting a vector
-joining the vector and DNA, using ligase to seal
-transform a host organism
-select host cells that have cloning vector
Correct Answer--made by bacteria
-cleave DNA at specific recognition sites
plasmid Correct Answer--aka a vector
-a circular DNA molecule that reproduces separately from the
chromosome, contains nonessential gens picked up from the
environment
transformation Correct Answer-the process by which plasmids are
introduced into bacterial cells
selectable marker gene Correct Answer--used to tell which bacterial
cells have picked up the plasmid
-eg antibacterial resistance
, Origin of replication Correct Answer-sequence in the plasmid where
replication is initiated
-required to propagate the plasmid
polylinker Correct Answer-place near promoter on a plasmid where the
GOI is inserted
transcription-termination sequence Correct Answer-a sequence that can
improve the quality and amount of mRNA produced
Why does translation need to be regulated during gene cloning? Correct
Answer-If the cell makes too much of the protein, the increased
concentration of protein can kill the cells
oligonucleotide directed mutagenesis Correct Answer--use a plasmid
with a targeted start site fo the mutation
-denature and add primers with the mutation in them
-use DNA pol to replicate plasmid and incorporate primer inside
-digest non mutated parent DNA templates
-transform dsDNA (w/mutant)
How are primers synthesized Correct Answer--first nucleoside is bound
to silica support
-next nucleotide is added
-repeat until all nucleotides are added
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller cracker. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $14.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
67096 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now