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BTC 195 Test Questions & Answers 2024/2025 $7.99   Add to cart

Exam (elaborations)

BTC 195 Test Questions & Answers 2024/2025

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BTC 195 Test Questions & Answers 2024/2025

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  • August 9, 2024
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  • 2024/2025
  • Exam (elaborations)
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  • BTC 195
  • BTC 195
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BTC 195 Test Questions & Answers
2024/2025

what assay did we use in lab for protein determination - ANSWERSbradford assay



coomassie blue dye - ANSWERSbinds to side chains of specific amino acids



what does the intensity of blue correlate with - ANSWERSconcentration of protein



how is protein concentration measured - ANSWERSby eye or using a spectrophometer



beers law - ANSWERSis solute absorbs light of a particular wavelength, the absorbance is directly
proportional to concentration of solute



equation used to prepare protein standards - ANSWERSM1V1=M2V2



another equation to measure protein standards - ANSWERSC1V1=C2V2



contain aromatic amino acids that absorb UV light at a wavelength of 280nm - ANSWERStyr, trp, phe, his



at what wavelength do proteins have a strong absorbtion at - ANSWERS280nm



Beer's Law equation - ANSWERSA=ecl



A - ANSWERSabsorbance: the readout you get



e - ANSWERSextinction coefficent: how well the molocule absorbs that wavelength

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