D) To determine concentration and mass of a protein - ANS-Purpose of generating standard
curve is to is to?
A) Determine unknown mass of a protein
B) Determine the unknown concentration of a protein
C) To determine concentration and mass of a protein
D) None. It's to torture students with math in a biology class
B) P20 - ANS-To measure out 20ul of sample, you will need to use ________ pipetmen :
A) P10
B) P20
C) P200
D) 1000
C) Spectrophotometer - ANS-__ measures amount of component in solution based on amount
of light absorbed
A) UV spectrometer
B) Spectrometer
C) Spectrophotometer
D) Specimen
A) 260, 280 - ANS-Nucleic acids absorb maximally at ___ nm while proteins absorb maximally
at ____ nm.
A) 260, 280
B) 280,260
C) 280,260
D) 595, 260
C) Bradford and Coomassie - ANS-_________ protein assay was performed to measure the
concentration of protein in a solution
A) Bradford
B) Coomassie
C) Bradford and Coomassie
D) None of the above
, D) To create range and determine protein's concentration - ANS-What was the purpose of
creating serial dilutions?
A) To determine protein's concentration
B) To create a range of absorbance
C) For pipetting practice
D) To create range and determine protein's concentration
B) Reddish-brown - ANS-Color of Coomassie dye when no protein is present ?
A) Blue
B) Reddish-brown
C) Greenish-brown
D) Clear
D) All of the above.
* It depends on the pH! - ANS-In what form does Coomassie dye exist in?
A) Positive, dependant upon pH
B) Neutral, dependant upon pH
C) Negative, dependant upon pH
D) All of the above.
C) Blue - ANS-Coomassie dye has this color when it has negative charge?
A) Red
B) Green
C) Blue
D) All of the above
True - ANS-True or False: Amt of blue is directly proportional to the amt of protein in solution
D) Protein and Colormetric - ANS-What type of assay is Coomassie assay?
A) Protein
B) Colorometric
C) Ribosomal
D) Protein and Colorometric
False - ANS-True or Flase: The more protein in solution, the darker brown coomassie will
appear
D) 1000 ul - ANS-1 milliliter has how many microliters?
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