bioc LAT2; Questions with Complete Solutions
Select the BEST answer.
The percentage oxyhaemoglobin in a haemoglobin solution can be calculated by:
using the ratio of absorbances of oxy- and deoxyhaemoglobin at wavelengths where the biggest
difference in absorbance readings are observed between...
bioc LAT2; Questions with Complete Solutions
Select the BEST answer.
The percentage oxyhaemoglobin in a haemoglobin solution can be calculated by:
using the ratio of absorbances of oxy- and deoxyhaemoglobin at wavelengths where the biggest
difference in absorbance readings are observed between the two forms.
adding a live yeast suspension to an oxyhaemoglobin solution and observe the change in
absorbance by spectrophotometry.
measuring the absorbance of an oxyhaemoglobin solution using the wavelength that
corresponds to its absorption maxima and then apply the Beer-Lambert formula.
measuring the absorbance of a haemoglobin solition at 560 nm, which is deoxyhaemoglobin's
absorption maxima and the wavelenth where oxyhaemoglobin absorb light the weakest.
Correct answer-using the ratio of absorbances of oxy- and deoxyhaemoglobin at
wavelengths where the biggest difference in absorbance readings are observed between the
two forms.
Absorbance may be defined as:
the ratio of lightpath length to incident light
the log of the ratio of incident light to transmitted light
, bioc LAT2; Questions with Complete Solutions
the ratio of concentration to transmitted light
the ratio of molar absorbance coefficient to lightpath length Correct answer-the log of
the ratio of incident light to transmitted light
Beer's Law implies that:
the concentration of a solution is dependent on lightpath length
the distance light travels through a solution is independent of concentration
the molar absorbance coefficient is dependent on the concentration of the absorbing solute
the absorption of light increases with increasing solute concentration Correct answer-the
absorption of light increases with increasing solute concentration
When haemoglobin in oxygenated in the lungs it is most likely to: Correct answer-
A 3 mL cuvette was filled with 0.5 mL of solution Y and 2.5 mL of water and its absorbance at
340 nm was 0.394.
The molar absorbance coefficient of solution Y is 31,000 L/mol/cm. The pathlength is 1 cm.
, bioc LAT2; Questions with Complete Solutions
What is the concentration of the diluted solution Y?
Express your answer in μM and with two decimal points. Correct answer-12.71
A spectrophotometric experiment yields the following data of a 1 in 3 diluted sample:
c = 2.3 mM
A = 0.46
l = 5 mm
wavelength = 480nm
What is the molar absorbance coefficient of the compound that was tested?
Provide your answer to the nearest whole number. Units are not required Correct
answer-400
Solution A had an initial concentration of 80mg/mL and after it had been diluted it had a
concentration of 4mg/mL. Which statement is INCORRECT regarding this dilution?
The dilution factor is 20.
It could be made by mixing 2 mL of solution A with 40 mL of water.
The concentration of the undiluted sample is 20 times greater than that of the diluted sample.
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