Biosafety Levels; Questions & Answers 100%
Verified
BSL 1 Correct answer-microorganisms not known to cause disease in healthy adults;
standard microbiological practices are followed, PPE is not required, a sink must be available for
hand washing; examples are bacillus subtilis, serratia marcescens, staphylococcus saprophyticus
BSL 2 Correct answer-indigenous microorganisms that can lead to diseases of varying
severity in healthy adults; BSL 1 procedures plus lab access is restricted, PPE must be worn
including lab coats, gloves, and eye protection, work that may generate aerosols or splashes are
performed in a biological safety cabinet, contaminated waste is autoclaved, the lab has self
closing doors, a sink and eye wash are available; examples: s. aureus, streptococcus pyogenes,
and salmonella
BSL 3 Correct answer-indigenous or exotic microorganisms that cause serious or
potentially lethal disease through respiratory transmission; BSL 2 procedures plus medical
surveillance of lab employees, immunizations may be required; locking, double door access
separates the labs work area from the other part of the facility, PPL must be worn including lab
coats, gloves, eye protection, and respirators; all work is performed in biosafety cabinet,
decontamination of all waste and lab clothing before laundering; lab must have negative air
flow, exhaust air cannot be recirculated; hands free sink and eye wash must be available;
examples: mycobacterium tuberculosis, bacillus anthracis, francisella tularensis
BSL 4 Correct answer-microorganisms that are dangerous and exotic with high risk of
aerosol transmitted infections; rarely are there treatments or vaccines for these
microorganisms, and the diseases they cause are frequently fatal; BSL 3 procedures plus
changing clothes before entering lab, shower upon exiting lab, decontaminate all materials
before exiting lab, all work is done in class 3 biosafety cabinet or by wearing a full body, positive
, Biosafety Levels; Questions & Answers 100%
Verified
pressure suit, lab must have negative air flow, and exhaust air cannot be recirculated, the lab
must be in a separate building or in an isolated and restricted zone of the building
fungi, 1 Correct answer-morphology & biosafety level of aspergillus
positive rod, 1 Correct answer-gram stain, morphology & biosafety level of bacillus
cereus
positive rod, 1 Correct answer-gram stain, morphology & biosafety level of bacillus
megaterium
positive rod, 1 Correct answer-gram stain, morphology & biosafety level of bacillus
subtilis
negative rod, 1 Correct answer-gram stain, morphology & biosafety level of citrobacter
freundii
postive rod, 1 Correct answer-gram stain, morphology & biosafety level of clostridium
beijerinckii
positive rod, 1 Correct answer-gram stain, morphology & biosafety level of clostridium
sporogenes
