____________ and ____________ are required for heme synthesis - iron and protoporphyrin
Fe Transport protein - Transferrin
Major Fe storage form - Ferritin
H2O insoluble Fe storage form (long-term) - Hemosiderin
Excess iron that is stored in tissues and body organs can lead to ____________ and ____________ -
hemosiderosis and hemochromatosis (organ damage)
What are the heme precursors (in order) - Delta, Porphobilinogen, UR, COP, PROTO
EDTA chelates ____ - Calcium
What are some sources of error for hemoglobin? - icterus, lipemia, hemolysis, increased white count
What are the hemoglobin reference ranges for men and women? - men: 16 +- 2 g/dL
women: 14 +- 2 g/dL
What happens in methemoglobin? - Fe2+ is oxidized to Fe3+, cannot bind O2, is brown
Measures packed cell volume in a percent - hematocrit
What are some sources of error from a calculated hematocrit? - cold agglutinins, high white count
, What are the hematocrit reference ranges for men and women? - male: 47 +- 5 %
women: 42 +- 5%
What is the calculation for MCV? - (HCT/RBC) x 10
What is the calculation for MCH? - (HB/RBC) x 10
What is the calculation for MCHC? - (HB/HCT) x 100
What is the reference range for MCV? - 80 - 100 fL
What is the reference range for MCH? - 28 - 32 pg
What is the reference range for MCHC? - 32 - 36 %
What indicates anisocytosis? - if the RDW is greater than 14.5%
What are the red cell reference ranges for men and women? - men: 4.5 - 6.1 E6/uL
women: 3.8 - 5.2 E6/uL
What stain do we use for a blood smear exam? - wright stain (romanowsky, eosin, methylene blue, and
methanol)
What white cell would be increased? Bacterial infections - neutrophils
What white cell would be increased? viral infections - lymphocytes
What white cell would be increased? Tb, syphillis, malignancies - monocytes
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