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SLCC Microbiology LAB Midterm Questions and Answers Fully Solved
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SLCC Microbiology LAB Midterm
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SLCC Microbiology LAB Midterm3 Methods Classify Bacteria - answer Morphology
Staining
Biochemistry
Coccus - answerA spherical-shaped bacterium.
Bacillus - answerRod shaped bacterium
Squiggly lines - answer spirochetes, spirilla
Strep - answerchain of bacteria
Staph - answergrape-like cluster
Hesse - answerused Agar instead of gelatin so organism wouldn't break it down
Petri - answerused smaller dishes for ease of use
Leeuwenhoek - answerA glass grinder who documented smaller organisms under his
homemade microscope
Koch - answeridentified the significance of a Pure Culture
Culture Medium - answerLiquid-Broth
Semi-solid solution <1% Agar
Solid >1% Agar
Culture Media - answerSlant
Stab
Gelatin Stab
Broth
Petri
Slant Tube - answer2% Agar Solution at a 40-60 degree slant
Deep tube - answer> 1% Agar solution in a tube
Gelatin Stab - answerused to test if an organism has an enzyme for gelatinase.
, Petri Dish - answer>1% Agar, lid larger than the base-stored upside down to prevent
condensation
Broth - answerno solidifying agents. Used to help increase sample through dilution
Can add pH indicators
Can add carbohydrates to test for metabolism properties.
Sterilization - answerHeat: Dry, open flame, moist (autoclave)
Filtration
Chemicals-antisepsis and disinfectants
Radiation: UV Rays, or gamma ionization
Transfer Tools - answerInoculation loops
Transfer Needles
Sterile Swabs
Various pipettes
L-shaped bent spreading rod
Cultivation - answerIncubators
Refrigerators
Freezers-storage
Subculture - answermeans to transfer one media to another
Pure Culture - answerseparation so you only have 1 type of organism to study in depth
Streak Plate - answerIsolation and dilution-try to get a single colony isolated from the
rest
Spread - answerused to
quantify
or
identify a susceptibility to antibiotics
Pour Plate - answerQuantitative
Aseptic (sterile) Techniques - answerPrevent CONTAMINATION-or at least try to
reduce
Abundance of Growth - answerNone
Slight
Moderate
Large
Pigmentation - answerColor
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