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MB ASCP Study Exam Questions With Correct Answers 2024
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MB ASCP Study Exam Questions With Correct Answers 2024
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MB ASCP Study Exam Questions WithCorrect Answers 2024
What uis umetagenome? u- ucorrect uanswer.Sampling uthe ugenome usequence uof ua
ucommunity uof uorganisms.
Polymorphism uinvolved ufor uIrinotecan? u- ucorrect uanswer.UGT1A1
NGS urelated uto udeletions uand uinsertions, ucoverage, udepth? u- ucorrect uanswer.-Paired-
end ureads ufor udetection uof uindels
-Coverage uis uhow umuch uof uyour utarget uhas ubeen uread
-Depth uis uhow umany utimes ua uunique uposition uhas ubeen ucovered
Polymerase ufor ulagging ustrand? u- ucorrect uanswer.Euk u- uDNA uPol uAlpha
Pro u- uDNA uPol uI
Polymerase ufor uleading ustrand? u- ucorrect uanswer.Euk u- uDNA uPol uDelta uand uDNA uPol
uEpsilon
Pro u- uDNA uPol uIII
Polymerase uused uin uLAMP? u- ucorrect uanswer.-Loop-mediated uIsothermal uAmplification
uutilizes uBst uPolymerase u
-Has uhelicase uactivity uand uactivity uat uannealing/elongating utemps
Highest uresistance ugene uin uenterococcus? u- ucorrect uanswer.vanA uhas uthe uhighest
uresistance uin uenterococcus
Resistance uin umecA ugene uin uMRSA ucodes ufor? u- ucorrect uanswer.mecA uresponsible ufor
uantibiotic uresistance uin uS. uaureus u(MRSA), uand ucodes ufor uPBP2a, uwhich uhas ulow
uaffinity ufor ubeta-lactam uantibiotics ulike upenicillin.
Important uin ugene uexpression, udefense uin ueukaryotes? u- ucorrect uanswer.Person
uguessed umRNA u- ugene-expression uand ucodes ufor uproteins uthat ubind uto uDNA uto
upromote/inhibit uexpression.
Donor/Recipient u- uwhat's uinformative uloci? u- ucorrect uanswer.No umatching ualleles uis
uinformative, uyou ucan utell uD ufrom uR; unon-informative uwould ube uboth uD&R umatching.
, Bond ubetween uDNA uand uhistones? u- ucorrect uanswer.Ionic u- uDNA uis unegative uand
uhistones uare upositive
Amino uAcids uthat umake uup uhistones? u- ucorrect uanswer.Arginine uand uLysine u- upositively
ucharged uamino uacids
Multiplex umodifications ufor ulow uyield? u- ucorrect uanswer.Person uguessed uKCl.
Increasing uMg2+ ucould uincrease uyield. u
Decreasing ustringency uincreases uyield, ubut uincreases uchances uof umispriming
PCR utroubleshooting u- ucorrect uanswer.Increase uTemp u--> uIncrease uStringency
Increase uProbe uLength u--> uIncrease uStringency
Increase uProbe uComplexity u--> uIncrease uStringency
Lower uSalt uConc. u--> uIncrease uStringency
Remove uMg2+ u--> uIncrease uStringency
Less uTime u--> uIncrease uStringency
Add uFormamide u--> uLowers uStringency
Add uBovine uSerum u--> uLowers uStringency
Increase uProbe uConc. u--> uLowers uStringency
How udoes uan uamplification ucontrol uhelp? u- ucorrect uanswer.Differentiates ua utrue unegative
ufrom ua ufalse unegative
Amplification ucontrols uhave ua utarget uthat ushould ualways uamplify, ueven uif uyour usample
udoes unot uamplify
Best umolecular uassay ufor uCystic uFibrosis? u- ucorrect uanswer.CF uis ucaused uby ua u3b
udeletion: udelF508
RFLP, uPCR uand uSouthern uBlot uare uused.
What uto uuse uFISH ufor: u- ucorrect uanswer.PWS/AS ucan ube udetected uwith uFISH
Gaucher u- uSequencing uor uenzyme uactivity uscreening
FXS u- uRFLP, uPCR, uSSCP
TS u- uSequencing uor uenzyme uactivity uscreening
How uoften ushould ua ucompetency ube ugiven/done ufor usomeone uin ua ucomplex ulab? u-
ucorrect uanswer.Annually
Important uin uFinal uLab uReport uin uCLIA? u- ucorrect uanswer.Lab udirector usignature?
Neurotoxin uin ugel ucomponents? u- ucorrect uanswer.Ethidium uBromide
What uphenomenon uis uobserved uin uEtBr uin ugel uelectrophoresis? u- ucorrect
uanswer.Products uslowed ubecause uEtBr ualters uDNA ushape, ucharge, uflexibility
Heteroduplex u- ucorrect uanswer.dsDNA uwhere uthe ucomplement ustrands uare unot
ucompletely ucomplementary
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