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Microbiology Lab UTA Final Exam With 100% Correct Answers
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Microbiology Lab UTA Final Exam With 100% Correct Answers
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Microbiology Lab UTA Final Exam With 100% Correct Answers How is Media made? - Correct Answer-5 g. T.S.A. and 125 mL Di water, cover flask with foil, affix autoclave tape, autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or stop when covers bottom.Agar - Correct Answer-linear polysaccharide polymer derived from agarose in red algae.
What does agar do? - Correct Answer-increases solidity, exhibits hysteresis- gels and melts at different temps. Melts above 85-90 and solidifies at 40-45.
What is liquid media? - Correct Answer-commonly called broths, doesn't contain agar, easy to use, allows observation of growth patterns.
Advantages to using semi-solid media - Correct Answer-To test oxygen requirements, to test motility. Fastidious - Correct Answer-only grows in a fortified culture media under specific conditions. The more _____________ the organism, the more specific ingredients a medium must have. Uses defined media.
Why use solid media? - Correct Answer-The maintenance and growth of stock cultures, to isolate single colonies, to ensure purity.
T.S.A. Plate - Correct Answer-Tryptic Soy Agar most
common medium used in lab. Undefined media that
contains casein digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of? - Correct Answer-Proteins,
Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of? - Correct Answer-70%
Autoclave - Correct Answer-sterilizes with steam at 121* C, pressure at 15 PSI for at least 15 min. Tested weekly to daily. Reservoir - Correct Answer-potential source of infection.
Normal commensal symbionts - Correct Answer-S. pneumoniae and s. aureus
Environmentally acquired microbes - Correct Answer-p. aeruginosa and b. cepacia
Magnifications of our microscopes - Correct Answer-scanning: 4x-40, low: 10x-100, high: 40x-
400, oil immersion: 100x-1000
What is resolving power? - Correct Answer-How close two objects can be and still be distinguished as separate.
What is the optimum resolution of the microscope using oil immersion? - Correct Answer-.2 micrometer.
How much does each microscope cost? - Correct Answer-$1600
convex - Correct Answer-Morphology of a domed colony raised - Correct Answer-flatter dome than convex
flat elevation - Correct Answer-
crateriform - Correct Answer-hole in convex colony
Umbonate - Correct Answer-Morphology of a colony
with a raised center
Rhizoid - Correct Answer-Morphology of a branching colony
lobate - Correct Answer-margin with lobes
undilated margins - Correct Answer-irregular but not lobed
curled - Correct Answer-wave pattern on margin
entire margin - Correct Answer-even smooth all around
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