Chap 11 Questions and Answers
An immunoassay is performed in the following manner to look for an antigen (Ag) in the patient serum: Patient serum + labeled Ag + known antibody (Ab) are added together and incubated. Unbound material is washed off. The amount of labeled Ag is then measured. What ass...
Chap 11 Questions and Answers
An immunoassay is performed in the following manner to look for an antigen (Ag) in the patient serum: Patient serum + labeled Ag + known antibody (Ab) are added together and incubated. Unbound material is washed off. The amount of labeled Ag is then measured. What assay is performed? - answer Competitive
Best describes competitive binding assays - answer A limited number of binding sites are present
identity the characteristics associated with non competitive enzyme immunoassays - answer Antibody is bound to the solid phase
Best describes homogenous assays - answer A washing step is not necessary
Testing situation where there is a direct relationship between the amount of bound label and the amount of patient antigen present - answer 1)Competitive radioimmunoassay using labeled antigen 2)Competitive enzyme immunoassay using labeled antigen
3)Noncompetitive enzyme immunoassay using a second labeled antibody
You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice? - answer Wavelengths detected by microtiter plate reader
Which assay is the best choice for measuring small molecules, such as drugs and small
peptide hormones? - answer Enzyme multiplied immunoassay technique (EMIT)
Point of care testing is performed most easily using - answer Immunochromatographic assay
Compared with a capture assay, a radioimmunoassay is likely to be more advantageous
in terms of - answer Analytical sensitivity
The signal measured in a fluorescence polarization immunoassay is the - answer Orientation of emitted light waves
Rheumatoid factor can cause false positive results in which type of assay - answer Heterogeneous noncompetive
Extremely high analytical concentrations can cause false negatives results (hook effect) in which type of assay? - answer Heterogenous noncompetitive Statement about noncompetitive enzyme immunoassay is true - answer All patient antigen is allowed to react with binding sites
What compound is an appropriate label in chemiluminescent assay - answer Ruthenium derivative
In a capture assay to determine the presence of a viral antigen in a patient sample, which of the following would be on a solid phase - answer Unlabeled antibody
The use of vitamin of B7 in a delayed capture assay is found in which technique ? - answer Biotin avoiding labeling assay
An indirect ELISA assay was performed on a patients serum sample. What is the relationship of the amount of enzyme activity to the amount of patients antibody measured? - answer Directly proportional
An immunoassay is performed in the following way: Antigen is bound to a solid support. Patient serum is added, and patient antibodies bind to the antigen. The well is washed, and enzyme-labeled anti-immunoglobulin antibodies are added. The well is washed, and enzyme substrate is added. Enzyme activity is determined. Which immunoassay is described? - answer ELISA
When is the enzyme labeled antibody added in the sandwich immunoassay testing technique? - answer Right after incubation stage
The "sandwich" inthe sandwich immunoassay testing techniques is between: - answer 2 reagent antibodies
To detect low molecular weight analyzes, which immunoassay is best? - answer Homogenous immunoassay
A urine drug screen is performed using which technique below? (LO 9, T2) - answer Homogenous immunoassay
A out patient physicians office is investigating the addition of a convenient way to perform analysis for a commonly tested analyte where results are often needed before the physician enters the examination room. Which is most appropriate? - answer Rapid immunoassay
How is pregnancy testing performed at home? - answer Immunochromatography
------ is more sensitive?this assay is also sensitive enough to detect very low and very high analyte concentrations? - answer Heterogenous chemiluminescense
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