MCB3020 Exam 3-Questions with Correct Answers/ Verified/ 100% Pass
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Course
MCB3020
Institution
MCB3020
reservoir - The site or natural environmental location in which the causative organism is
normally found is called the _________.
Herd - ___________ immunity is an epidemiological concept that explains the resistance
of a population to infection and the spread of an infectious organism due to th...
MCB3020 Exam 3 -Questions with Correct Answers/ Verified/ 100% Pass Reverse transcriptase process - ✔✔1. poly -T primer to single -stran d RNA 2. reverse transcriptase to produce DNA -RNA hybrid 3. enzyme RNaseH to cut RNA and regenerate RNA primers 4, DNA polymerase and ligase synthesizes new complementary DNA strand cloning vector - ✔✔allows for transfer of gene of interest to a host orga nism; allows for the making of recombinant DNA good cloning vector qualities - ✔✔origin of replication selectable marker (i.e. antibiotic resistance gene) unique restriction sites that allow the insertion of gene of interest types of cloning vectors - ✔✔plasmid (carry 20k base pairs) bacteriophage (carry 9k -25k base pairs) cosmid (carry 30k -47k base pairs) artificial chromosomes (carry up to 1 million base pairs) PCR ingredients - ✔✔primers Taq polymerase deoxyribonucleotide triphosphates PCR temperatures - ✔✔90 - separates DNA strands 50 - primers attach 70 - nucleotides are added on types of polymers for gel electrophoresis - ✔✔agrose polyacrylimide ethidium bromide - ✔✔stains DNA, makes it visible under UV RFLP Analysis procedure - ✔✔1. isolate gene of interest 2. PCR 3. cut DNA, making restriction fragments 4. gel electrophoresis to separate according to size 5. computers to analyze ribotyping - ✔✔compares rRNA to find similarities/differences across domains southern blotting - ✔✔1. cut DNA to make a lot of fragments 2. separate DNA by size via gel electrophoresis 3. place sheet of nitocellulose paper on top of gel 4. place paper in heat -sealed bag with radioactive probe 5. wash filter and place on x -ray film to make autoradiograph western blotting - ✔✔detects proteins northern blotting - ✔✔detects RNA making of a genomic library - ✔✔1. extract DNA 2. cut into fragments of specific size 3. insert the fragments into a vector 4. insert vector to host organism 5. DNA can now be amplified/retrieved site-directed mutagenesis - ✔✔1. identify sequence 2. complementary DNA sequence in lab with the mutation 3. insert the sequence into target cell 4. target cell swaps target sequence with complementary sequence with m utation protoplast fustion - ✔✔cells without cell walls are put together in solution, then shocked electrically to fuse them all together interferons - ✔✔antiviral molecules alpha, beta, gamma treats viral infections primary metabolite - ✔✔essential for proper growth, its removal significantly impacts survival secondary metabolite - ✔✔not directly involved in growth; its removal doesn't significantly impact survival; productions is usually triggered by exogenous events agricultural applications of gene tic manipulations - ✔✔plant hormones food additives genetically modified plants biopesticides chemostat - ✔✔bioreactor with a constant volume caused by a constant inflow of fresh medium and output of culture liquid industrial applications of genetic manipulations - ✔✔biopolymers (gels) biosurfactants biofuels (ethanol, methane gas, hydrogen gas) bioconversion biosensors
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