AAB MT Exam Hem Lab Methods
What type of specimen is used for hematology testing? - ANS-EDTA
What type of specimen is used for coagulation testing? - ANS-3.2% citrated plasma
When should a 3.2% citrated plasma be rejected? - ANS-If the specimen is clotted,
hemolyzed or QNS.
How is platelet poor plasma obtained from a 3.2 citrated plasma specimen? - ANS-By
centrifuging the specimen.
In a coagulation specimen, what is the ratio between anticoagulant and whole blood? -
ANS-1:9 ratio
In a coagulation specimen, what is the ratio between whole blood and anticoagulant? -
ANS-9:1 ratio
What is the formula for the Red Blood Cell (RBC) Count? - ANS-(Cells counted X
Dilution X 10) / Number of square millimeters counted
What is a common source of errors for the RBC Count? - ANS-Cold agglutinins (due to
cold antibodies present).
Why is platelet poor plasma essential for coagulation testing? - ANS-Platelets initiates
the coagulation cascade (results in shorten result times).
Cold auto-antibodies cases what errors in the CBC test? - ANS-A falsely decreased
HCT and RBC, plus a falsely elevated MCV and MCHC (greater than 37).
If a patient has a cold auto-antibody, what can be done to obtain a correct CBC? -
ANS-Incubate specimen at 37 C, reanalyze specimen immediately after incubation, and
immediately make the peripheral smear.
What is the method for performing a hemoglobin concentration? -
ANS-Cyanmethemoglobin method
What is the principle behind the cyanmethemoglobin method? - ANS-That hemoglobin +
ferricyanide yields cyanmethemoglobin
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