AAB MT Chemistry
Which of the four elements in proteins differentiates this class of substances from
carbohydrates and lipids?
Nitrogen
Which of the proteins is soluble in water?
Albumin
How are amino acids joined together to form a chainlike structure?
Peptide bonding
Amino acid sequence of a protein represent which level of protein structure?
Primary structure
The alpha helix and beta pleated sheet represent which level of protein structure?
Secondary structure
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The 3D structure of a protein represent which level of protein structure?
Tertiary structure
2 or more polypeptide chains represent which level of protein structure?
Quaternary structure
Protein classification that contains peptide chains only
Simple proteins
Protein classification that contains a protein and a prosthetic group (Lipoproteins,
Glycoproteins, Mucoproteins, Nucleoproteins)
Conjugated proteins
Protein classification for proteins that come off of an electrophoreisis
Plasma proteins
, Proteins may become denatured when subjected to mechanical agitation, heat, or chemical
treatment. What does denaturation of proteins refer to?
Alteration in tertiary structure
How many immunoglobulin classes are currently recognized? What are the
immunoglobulins?
5
- IgG
- IgA
- IgM
- IgE
- IgD
Glycoproteins and Mucoproteins are usually bound to which substance?
Carbohydrates
Which reagent is employed in the serum protein determination? This is the most common
method used for measurement of total proteins
Biuret
What are the different ways measure total proteins
- Kjeldahl
- Refractometry
- Biuret
- Dye binding
The measurement of nitrogen content in total proteins
Kjeldahl
The measurement of refractive index for total proteins
Refractometry
Different dyes used in the dye binding method for measurement of total proteins
- Bromphenol blue
- Ponceau S
- Amido black
- Coomassie brilliant blue
Largest fraction of proteins and first peak that comes off of the electrophoresis.
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