MLPAO Test Prep ~ Lab Safety and
Equipment
Volumetric pipette - ANS-Extremely accurate, calibrated to accurately deliver a fixed
volume of liquid
Serological pipette - ANS-Graduated pipette in which calibration marks extend to the tip
Mohr pipette - ANS-Graduated pipette in which calibration marks end at a point above
the tip
Most accurate pipette - ANS-Volumetric
What is the graduated cylinder used to measure - ANS-make accurate volume
measurements
What is more accurate a graduated cylinder or beaker? - ANS-Graduated Cylinder
Borosilicate glassware is used for it's high ___ and _____ resistance - ANS-temperature
and chemical
Polystrene is commonly used for _____ materials, is not autoclavable, and limited to
____ - ANS-disposable, solvents
Autoclave ideal temperature - ANS-121c for 15m at 15 psi
Biohazard waste for decontamination - ANS-Garbage cycle
Liquids and slow steam exhaustion - ANS-Liquid cycle
Dry glassware/Plastic - ANS-Dry Cycle
Bagged - ANS-Gravity cycle
Ideal temperature for dry heat - ANS-160c for 1h
Sterilization definition - ANS-Destruction of all microbial life including spores
, Disinfection definition - ANS-Elimination or reduction harmful microorganisms
Microscope: Magnification definition - ANS-Ability to increase the size or enlarge the
image (not clarity)
Microscope: Resolution definition - ANS-Ability to clearly differentiate and determine two
close objects as separate identities
Microscope: Refraction definition - ANS-The act of bending light as it passes from one
medium to another
Microscope: Refractive index definition - ANS-The speed at which light passes from one
medium of a particular density to another in a vacuum
Microscope: Numerical Aperture definition - ANS-Measure of its ability to resolve fine
details of an image
Microscope: Vernier scales definition - ANS-Relocate a specific object on a slide using
the scales located on the microscope stage
WHMIS classes and Hazard symbols: Hazards under Class A - ANS-Compressed Gas
WHMIS classes and Hazard symbols: Hazards under Class B - ANS-Flammable and
Combustible material
WHMIS classes and Hazard symbols: Hazards under Class C - ANS-Oxidizing
Materials
WHMIS classes and Hazard symbols: Hazards under Class D - ANS-Materials causing
Immediate and serious toxic effects (1), Materials causing other toxic effects (2),
Biohazardous infectious materials (3)
WHMIS classes and Hazard symbols: Hazards under Class E - ANS-Corrosive Material
WHMIS classes and Hazard symbols: Hazards under Class F - ANS-Dangerously
Reactive Material
Class of Compressed Gas - ANS-A
Class of Flammable and Combustible material - ANS-B
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