MCB 2010 Test 2 Review
Review characteristics of a simple microscope - ANS-1. lens
2. location of specimen on pin
3. specimen- positioning screw
4. stage was a positioning screw
5. used natural light
compare simple and compound microscopes - ANS-1. compound: use multiple lenses
linked by an optical tubes to magnify and focus images. Objective lens at one side of
the tube enlarges an image of the specimen using lenses that magnify 4x, 10x, 40x,
100x. each objective lens is color coded. ocular lens at the other side of the tube
demagnifies the image ten more times. it has a condenser lens below the stage that
focuses the light for brighter image and it has an iris diaphragm that adjusts the light for
proper contrast between the image and the background.
2. simple microscopes: only have one lens, use natural light, and have no condenser or
diaphragm.
ocular lens - ANS-eye piece that demagnifies the image formed by the objective lens
body tube - ANS-transmits the image from the objective lens to the ocular lens
arm - ANS-side pillar
objective lenses - ANS-primary lenses that magnify the specimen
stage - ANS-holds the microscope side in position
condenser - ANS-focuses light through the specimen
diaphragm - ANS-controls the amount of light entering the condenser
illuminator - ANS-light source
fine and course adjustment knob - ANS-move stage up and down
base - ANS-bottom of the microscope
Antoni Van Leeuwenhoek - ANS-invented the simple microscope
, Robert Hooke - ANS-invented the first compound microscope and condenser (ball of
water)
Louis Joblot - ANS-first to put together all the pieces of microscope using side pillar
(arm) and iris diaphragm.
Aspects of magnification - ANS-1. objective lens power (4,10,40,100)
2. ocular lens power: 10x
3. objective lens power x ocular lens power = total magnification
4. objective lens always present in microscopy
magnification - ANS-has the power to reveal new details because of a higher resolution
magnification - ANS-as magnification increases, aperture size decreases, resolution
increases, working distance decreases and more details are revealed
Name objective lens - ANS-1. red: 4x: scanning
2. yellow: 10x: low power
3. blue: 40x: high power
4. white: 100x: oil immersion
how to calculate total magnification - ANS-ocular lens power x objective lens power
resolution - ANS-the ability of an optical instrument to make more details visible,
allowing individual parts of an image, or very close objects, to be seen separately by
spacing them out. it also determines smallest size visible to the normal human eye and
limits how sensitive the eye is to extremely fine details.
useful magnification - ANS-any increase in magnification that produces a higher
resolution by revealing new details.
empty magnification - ANS-magnification that releases no more details or improves
resolution
limits of resolution - ANS-1. human eye: 200 um
2. light microscope: 200 nm
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