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ASCP MOLECULAR BIOLOGY 2024 LATEST UPDATE CERTIFIED STUDY GUIDE RESOURCES COMPREHENSIVE QUESTIONS AND VERIFIED ANSWERS [MOST TESTED QUESTIONS] GET IT RIGHT GRADE A+ $15.99   Add to cart
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ASCP MOLECULAR BIOLOGY 2024 LATEST UPDATE CERTIFIED STUDY GUIDE RESOURCES COMPREHENSIVE QUESTIONS AND VERIFIED ANSWERS [MOST TESTED QUESTIONS] GET IT RIGHT GRADE A+

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ASCP MOLECULAR BIOLOGY 2024 LATEST UPDATE CERTIFIED STUDY GUIDE RESOURCES COMPREHENSIVE QUESTIONS AND VERIFIED ANSWERS [MOST TESTED QUESTIONS] GET IT RIGHT GRADE A+

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  • June 15, 2024
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  • 2023/2024
  • Exam (elaborations)
  • Questions & answers

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  • ASCP MOLECULAR BIOLOGY
  • ASCP MOLECULAR BIOLOGY

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ASCP MOLECULAR BIOLOGY
2024 LATEST UPDATE


CERTIFIED STUDY GUIDE RESOURCES
COMPREHENSIVE QUESTIONS AND VERIFIED
ANSWERS [MOST TESTED QUESTIONS] GET IT
RIGHT GRADE A+


What molecular methods can be used to validate the PCR
products ? - (correct answer) Electrophoresis and
Sequencing


What does DNA sequence analysis do? - (correct answer)
Direct determination of the order, or sequence, of nucleotides
in a DNA polymer.


What does it mean when you have dye blobs running the
sequencing? - (correct answer) It means that you fail to
clean the sequencing ladder properly which can result in bright
flashes of fluorescence that obliterate parts of the sequence
read.

, What if you have too many ddNTP in a DNA sequencing run?
too low of ddNTP? - (correct answer) Too many ddNTPs will
result in a short read. Too low will result in loss of sequence
data close to the primer but give a longer read.


How does the Sanger Sequencing work? - (correct answer)
For electrophoresis, what are some factors that determine the
mobility of molecules? - (correct answer) size of the
molecule and its conformation, the net charge of molecule,
temperature, and the pore size of matrix


Long DNA can retard the mobility of fragment, so how does the
pulsed-field electrophoresis overcome this? - (correct answer)
The voltage gradient is periodically re-oriented which helps
overcome this.


What is blotting? - (correct answer) Blotting is the transfer
of fractionated DNA from the gel to a solid support such as a
nylon membrane for further hybridization.


What is Northern blot? - (correct answer) Northern blot is
an extension of Southern blot that uses RNA instead of DNA for
testing.

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