Chapter 02 - Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis
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Microbiology (MICROBIOLOGY)
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Abacus College, Oxford
Chapter 02 - Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis /Chapter 02 - Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis
methods for the culturing and microscopic analysis
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Chapter 02 - Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis
2-1
Chapter 02
Tools of the Laboratory: Methods for the Culturing and Microscopic
Analysis
Multiple Choice Questions
1. The Five I's of studying microorganisms include all of the following except
A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based,
molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Section: 02.01
Topic: Culturing Microorganisms
Topic: Identifying Microorganisms
2. The term that refers to the purposeful addition of microorganisms into a laboratory
nutrient
medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
,Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Section: 02.01
Topic: Culturing Microorganisms
3. A pure culture contains
A. only one species of microorganism.
B. only bacteria.
C. a variety of microbes from one source.
D. a variety of species from the same genus.
E. None of the choices is correct.
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Section: 02.01
Topic: Culturing Microorganisms
4. The correct microbiological term for the tiny sample of specimen that is put into a
nutrient
medium in order to produce a culture is the
A. colony.
B. inoculum.
C. streak.
D. loop.
E. None of the choices is correct.
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
,Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Section: 02.01
Topic: Culturing Microorganisms
5. Which of the following is essential for development of discrete, isolated colonies?
A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Learning Outcome: 02.02 Discuss three physical states of media and when each is used.
Section: 02.01
Topic: Culturing Microorganisms
6. Which method often results in colonies developing down throughout the agar along with
some colonies on the surface?
A. streak plate
B. spread plate
C. pour plate
D. All of the choices are correct.
E. None of the choices is correct.
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
, Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Section: 02.01
Topic: Culturing Microorganisms
7. What type of isolation technique is most effective for the majority of applications?
A. pour plate
B. streak plate
C. spread plate
D. loop dilution
E. culture plate
ASM Objective: 08.02 Use pure culture and selective techniques to enrich for and isolate
microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Outcome: 02.01 Explain what the Five I’s are and what each step entails.
Section: 02.01
Topic: Culturing Microorganisms
8.
Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled,
and cooled?
A. a pure culture
B. a mixed culture
C. a solid medium
D. a liquid medium
E. a contaminated medium
ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based,
molecular and serological).
ASM Topic: Module 08 Microbiology Skills
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