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Biology 242 LAB Exam 2 With Correct Questions And Answers 2024
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Biology 242 LAB Exam 2 With Correct Questions And Answers 2024
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Biology 242 LAB Exam 2 With Correct Questions And Answers 20243 physical requirements for microbial growth - CORRECT ANSWERS-temp, pH, osmotic pressure
agar deep - CORRECT ANSWERS-motility and O2 requirement
agar plate - CORRECT ANSWERS-isolated colonies
agar slant - CORRECT ANSWERS-transport and sustaining of cultures
agar? and what temp does it melt? - CORRECT ANSWERS-a gel-like polysaccharide compound used for culturing microbes; extracted from certain red algae; 100C
Alcohols - CORRECT ANSWERS-(Ethanol, Isopropanol) denature proteins (requires water), dissolve lipids. Can enhance effectiveness of other agents, used to degerm skin.
Antisepsis - CORRECT ANSWERS-Removing pathogens from living tissue
Asepsis - CORRECT ANSWERS-ABSENCE of contamination
Autoclave - CORRECT ANSWERS-· used for sterilizing culture media instruments, dressings, intravenous equipment, etc.
· Uses steam heat
· Autoclaves are more effective than hot air for sterilization
autotrophs - CORRECT ANSWERS-make there OWN food
Bacteriostasis - CORRECT ANSWERS-inhibiting growth, not killing microbes
Biguanides - CORRECT ANSWERS-(Chlorhexidine) disrupt plasma membranes, combined with detergent or alcohol in surgical scrubs
binary fission - CORRECT ANSWERS-organism separates into two new organisms and
splits cytoplasm evenly
Biofilms - CORRECT ANSWERS-bacteria grouped together in communities that provide
great BENEFITS from that grouping.
Bisphenols - CORRECT ANSWERS-· : (hexachlorophene, triclosan) disrupt plasma membranes broth - CORRECT ANSWERS-liquid media used for growing cells
Budding method - CORRECT ANSWERS-new organism is from old organism and cytoplasm splits unevenly
carbon cycle - CORRECT ANSWERS-o Carbon moves between the atmosphere and the ocean through diffusion.
o Aquatic plants absorb carbon through photosynthesis.
o Terrestrial plants remove carbon from the atmosphere through photosynthesis.
o Fish and other marine life and animals obtain carbon through consumption (heterotrophs); energy and CO2 released through respiration.
o Carbon is returned to water and air through respiration.
o When organisms die, carbon is decomposed and transferred through the ocean floor by sedimentation.
catalase - CORRECT ANSWERS-An enzyme produced in all cells to decompose hydrogen peroxide, a by-product of cell respiration
chemically defined media - CORRECT ANSWERS-growth of chemoautotrophs and photoautotrophs; microbiological assays
Colony - CORRECT ANSWERS-a population of cells arising from a single cell or spore (or from a group of attached cells)
complex media - CORRECT ANSWERS-growth of most chemoheterotrophic organisms
(EX:nutrient broth/agar)
Culture - CORRECT ANSWERS-microbes growing in/on culture medium
culture medium/media - CORRECT ANSWERS-Sterile (nothing growing yet) nutrient food source for growth of microorganisms
danger zone for food - CORRECT ANSWERS-15° to 52° C, 60° to 130° F
DEATH CURVE: Bacterial populations die at a constant logarithmic rate. - CORRECT ANSWERS-One log decrease= 90% of population killed
Death phase - CORRECT ANSWERS-least susceptible to antibiotics
Desiccation - CORRECT ANSWERS-· absence of water (extreme drying), prevents metabolism
differential media - CORRECT ANSWERS-differentiation of colonies of desired microbes from others
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