1. Replication of DNA begins at a single origin of replication and
then proceeds the entire length of the strand, in one direction
(T/F) - ANS -False
2. What enzyme is responsible for relieving the stress associated
with the twisting and untwisting of a DNA strand during
replication. - ANS -Topoisomerase
3. As a replicated DNA strand elongates, what enzyme is
responsible for adding nucleotides? - ANS -DNA polymerase
,4. The energy required to add a nucleotide to a growing chain is
supplied by an exergonic reaction involving the hydrolysis of the
nucleotide's pyrophosphate tail (T/F) - ANS -True
5. During elongation, in which direction are new nucleotides being
added to the leading strand? - ANS -5' —> 3'
6. The leading strand requires ____ # of RNA primers, and the
lagging strand requires ____ # of RNA primers. - ANS -One; more
than one
7. What replaces RNA nucleotides with DNA nucleotides in a newly
synthesized strand? - ANS -DNA Polymerase
, 8. A class of DNA polymerase patrols newly added nucleotides and
will remove nucleotides that are erroneous. How does the
polymerase "know" a nucleotide is misplaced. - ANS -Newly adde
nucleotides are compared to the complimentary template strand.
9. Mismatch repair is the process of identifying nucleotides that
are inappropriately matched, such as guanine with a thymine,
and removes the incorrect nucleotide from the newly
synthesized strand (t/f) - ANS -True
10. Nucleotide excision repair (cutting DNA) is accomplished by
what enzyme? - ANS -Nucleases
11. Telomeres contain a large proportion of vital coding DNA
(T/F) - ANS -False
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