MLPAO Test Prep ~ Microbiology
Microbiology is the study of - ANSMicroscopic organisms
Four major groups of Microorganisms - ANSBacteria, Viruses, Fungi, Parasites
Viruses are non cellular - ANSParasites
Viruses are observed through - ANSElectron microscope
Viruses contain a genomic core made up of either ____ or ____ (never both!) and a coat of
____ surrounding the core called an ___ - ANSDNA, RNA, protein, envelope
Viruses are obligate intracellular_____ - ANSParasites
Prokaryotic bacteria ranges in size between ____ um - ANS2-6
Eukaryotic fungi size ___ um - ANS2-100
Unicellular fungi include - ANSyeast
Multicellular fungi include - ANSMolds, fungi, Candida
Eukaryotic protozoa ranges in size between ___ um - ANS5-200
Protozoa are motile by means of - ANSpseudopods, cilia, or flagella
Microorganisms from largest to smallest - ANSProtozoa, fungi, bacteria, viruses
A form of providing all necessary nutrients to the microbe in an artificial environment is -
ANSGrowth medium
Growth Medium: Prepared by using ___ ____ or using _____ _____ - ANSindividual
constituents, dehydrated powders
Growth Medium: Classified according to three properties - ANSPhysical, Chemical, Functional
Growth Medium: Physical classifications - ANSLiquid, semi-solid, solid
Growth Medium: Chemical classifications - ANSSynthetic, non-synthetic
, Growth Medium: Functional classifications - ANSGeneral purpose, enriched, selective,
differential, anaerobic, enumeration, assay, specimen transport
Growth Medium: Solid medium gives a firm surface on which - ANSbacteria can grow
Growth Medium: Cells that grow on solid media are - ANSColonies
Growth Medium: A colony is produced of a ___ and is made of _____ - ANSsingle cell, one type
of species
Growth Medium: A colony's ____, ____, and ____ are typically used for identification -
ANSSize, shape, color
Growth Medium: Semi-solid medium is used to asses the ____ of bacteria or observe a ____ -
ANSmotility, reaction at a specific site
Growth Medium: Semi-solid medium contains _____ agents to thicken the media such as ____
or ____ - ANSSolidifying, gelatin, agar
Growth Medium: Liquid medium includes _____ or _____ - ANSBroths, infusions
Growth Medium: Liquid medium growth appears ____ the tube in the form of ____, _____, or
_____ appearance - ANSThroughout, dispersed, cloud, particle
General purpose medium grows a _____ _____of microbes - ANSwide variety
General purpose medium is considered to be _____ and contain a variety of nutrients to support
a wide range of microbes (e.g.. MH) - ANSnon-synthetic
Enriched Medium: Media is prepared with ____ _____ ____ (e.g.. BA) - ANSadditional growth
factors
Enriched medium is used to _____ and ____ organisms that are unable to grow on ____ media
and display _______ reactions - ANSrecover, investigate, basic, hemolysis
Enriched medium is prepared with ____% sheep blood added to ____ base - ANS5, TSA
Differential medium as it is also used to analyze bacteria that produce _____ - ANShemolysin
Beta hemolysis is ____ hemolysis and has a ______ appearance of agar around the colonies -
ANScomplete, clear
Alpha hemolysis is ____ hemolysis and has a _____ appearance of agar around the colonies -
ANSpartial, greenish (discoloration)