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MLPAO Test Prep ~ Hematology 2024/2025 graded A+ by experts
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MLPAO Test Prep ~ Hematology 2024/2025 graded A+ by experts
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MLPAO Test Prep ~ HematologyWhat does CBC stand for? - ANSComplete Blood Count
What are the formed elements of blood? - ANSWBC, RBC, and Platelets
EDTA Anticoagulant - ANSMaintains cell shape and size and prevents blood from clotting by
binding to calcium
Heparin Anticoagulant - ANSInitiates cell clumping and provides blue background staining
Citrate Anticoagulant - ANSDilutes the blood due to the volume of the liquid and is used for
platelet count only
CBC handling and storage: Refrigerate at __ᵒC until testing - ANS4
CBC handling and storage: When transporting to other labs/hospitals - ANSsurround with ice
packs
CBC handling and storage: Samples are _____ before testing - ANSmixed well by inversion
CBC handling and storage: Samples must be tested within __ - ANS24h
CBC handling and storage: Peripheral blood smears must be made within - ANS24h
CBC handling and storage: Samples kept for longer than ___ = CANNOT BE USED FOR
TESTING - ANS24h
What happens if CBC is tested after 24h? - ANSPlatelets clump, WBC nuclei disintegrate, WBC
cytoplasm contain dark granules and Vacuoles, and RBCs swell and break down
Coagulation: Specimen of choice? - ANSPlasma
Coagulation: What colour tube? What is the additive? - ANSlight blue, sodium citrate
Sodium Citrate binds to ___ preventing ____ - ANScalcium, coagulation
Sodium Citrate tube must be - ANSfilled to the maximum draw capacity
Coagulation: Document if patient on - ANSanticoagulant therapy or aspirin
, Handling and Storage of Coagulation samples: Preparation requirements - ANSCentrifuge and
separate
Handling and Storage of Coagulation samples: ____ plasma if not tested within ___ of arrival -
ANSFreeze, 4h
Coagulation samples: Some clotting factors are considered to be - ANShighly labile (destroyed
at room temperature)
What are the three methods of Peripheral blood smears (PBS)? - ANSWedge method (manual),
Spun method (automated slide spinner), and automated method (analyzer)
Thickness of film: HEMOGLOBIN/HEMATOCRIT INCREASED = angle of the spreader slide ___
- ANSDecreased
Sodium Citrate tubes should be drawn ___ except when blood cultures are ordered. - ANSFirst
Thickness of film: HEMOGLOBIN/HEMATOCRIT DECREASED = angle of the spreader slide
___ - ANSIncreased
HCT Values: Male, Female - ANS0.40-0.50, 0.35-0.59
Good blood film should be ___ to ___ the length of the slide - ANS2/3, ¾
Good blood film should have a slightly ___ or ____ edge - ANSRounded, feathered
The angle of the spreader slide is approximately ___ degrees - ANS35
The greater the angle of the spreader slide the ____ and _____ the film - ANSShorter, Thicker
The lower the angle of the spreader slide the ___ and ____ the film - ANSLonger and Thinner
Blood film should be allowed to ____ ___ completely before staining procedures - ANSair dry
Fixative found in stain is - ANSMethanol
Slides are washed with buffer of pH ___ to develop colour on the slide - ANS6.4
Stain is comprised of ____ and ____ dyes - ANSBasic, acidic
What is the basic (cationic) dye? - ANSMethylene blue
Methylene blue stains the ___ of the cell and the granules of _____ (____ in colour) -
ANSNucleus, basophils, blue-grey
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