Exam (elaborations)
Phlebotomy Exam 1 ( 100% guaranteed pass) 2024/2025
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Phlebotomy Exam 1 ( 100% guaranteed pass) 2024/2025
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Phlebotomy Exam 1Yellow Tube 1 - ANSBlood cultures
SPS
Aerobic bottle drawn first/anaerobic second
This tube is drawn first due to infection purposes
Use chloraprep for aseptic site
Mix 8-10 times
Lavender Tube 5 - ANSAnticoagulant
EDTA- sodium or potassium
Common tests- CBC
Prevents clotting by binding calcium
Prevents platelet clumping
Preserves shape of cell
NEVER BEFORE HEPARIN
Mix 8-10 times
Post prandial glucose test - ANSGlucose test performed after ingestion of a meal
Hematopoiesis - ANSblood cell formation; occurs in red bone marrow
Glycogen - ANSForm of glucose that muscles use for energy
Alimentary canal - ANSEntire tract from mouth to anus
occult blood test - ANSchemical test for the presence of blood in the feces that is not visible to
the eye
Light blue tube 2 - ANSAnticoagulant: sodium citrate
Prevents coagulation by binding calcium
Used for coagulation studies
Tests- PT or PTT
Special filling requirement (9:1 of blood to anticoagulant)
Light green/dark green tube 4 - ANSAnticoagulant: sodium heparin, lithium heparin, ammonia
heparin
Common tests- electrolytes
Prevents coagulation by inhibiting action of thrombin
Mix 8-10 times
Tiger top SST/Gold tube - ANSClot activatior
, Gel separator
SST- serum separator tube
Gray tube 6 - ANSContains and anticoagulant and preservative
Anticoagulant- potassium oxalate: binds calcium
Preservative- sodium flouride
Inhibits glycolytic activity of cells
Tests- alcohol levels
Mix 8-10 times
Pink tube - ANSAdditive- anticoagulant EDTA
Used for blood bank
Dark blue tube - ANSAdditive- either no additive, sodium heparin, or EDTA
Used for trace metal assays/toxicology studies
Note: tops are chemically cleaned to avoid stray traces of materials
Butterfly method - ANSAdvantages/ uses- smaller veins and tough sticks
Safety- device has button that retracts needle
Syringe method - ANSAdvantages- more control and collapsing veins
Evacuated tube method - ANSUsed in all 3 methods
Stores method immediately during/after draw
Additives in tubes have different actions on blood
Additives are usually either an anticoagulant or preservative
Plasma - ANSAll clotting factors
Liquid portion of blood that has NOT clotted
Serum - ANSNo clotting factors
Liquid portion of clotted blood
Preservative - ANSSlows metabolic activity of blood cells
Permits more time for testing
Evacuated tube method AKA straight needle - ANSDouble sided multi sample needle with
slanted bevel edge
Double sided: one needle punctures vein while other needle punctures tube
Can draw several tubes at one time
Safety features- cap to close needle
3 primary methods - ANSEvacuated tube method (straight needle)
Butterfly method
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