MICROBIOLOGY TEST 1 GALEN COLLAGE OF
NURSING (ACTUAL EXAM) LATEST 2024 WITH 130+
EXPERT CERTIFIED QUESTIONS AND ANSWER
ALREADY GRADED A+ 100% PASS
What are the 3 domains? - ANSWERS-Eubacteria
Archaea
Eukarya
What is phototrophy? - ANSWERS-Photosynthesis. Energy from light trapped and
converted to chemical energy. A two-part process, light reactions and dark
reactions.
Eukaryotic flagella and cilia - ANSWERS-Forms microtubles. Distinctly different
from prokaryotic flagella and cilia.
Archaea? - ANSWERS-prokaryotic, unicellular, no peptidoglycan in cell wall,
unusual metabolism.
Oxidative phosphorylation chemiosmotic theory - ANSWERS-ATP is synthesized as
the result of electron transport driven by the oxidation of a chemical energy
,source. Electron transport system creates the proton motive force (a high
concentration of protons on 1 side of the membrane)
Eukarya? - ANSWERS-- eukaryotic (protista- protozoa/algae, fungi- yeast, molds
and mushrooms, animals and plants), unicellular or multicellular.
Competitive inhibition - ANSWERS-Inhibitor competes with the normal substrate
for the active sites of enzymes
A pathogenic microbe is? - ANSWERS-Disease causing?
Flagella staining? - ANSWERS-A mordant is used on flagella. Carbolfuchsin simple
stain.
Explain bacteria. - ANSWERS-Prokaryotes, has peptidoglycan cell wall, various
energy sources: organic chemicals, inorganic chemicals, or photosynthesis.
Facilitated diffusion? - ANSWERS-Uses a transport protein
Explain archaea. - ANSWERS-Prokaryotes, no peptidoglycan, love in extreme
environments, unusual metabolisms. Includes methanogenes, extreme halophiles,
extreme thermophiles.
What is total magnification? - ANSWERS-Objective lens times ocular lens
,Krebs cycle - ANSWERS-Oxidation of acetylCoA produces NADH and FADH2.
What are special stains used for? - ANSWERS-Capsule stain, endospore stain,
flagella stain.
Anabolism? - ANSWERS-The synthesis of complex organic molecules from simpler
ones, requires ATP.
Simple stains? - ANSWERS-Uses a single basic died. A mordant may be used to
hold the stain or coat the specimen to enlarge it.
Metabolism is? - ANSWERS-The total of all chemical reactions in the cell and is
divided into two parts catabolism and anabolism.
What is oxidative phosphorylation? - ANSWERS-Energy released from transfer of
electrons, oxidation, of one compound to another, reduction, is used to generate
ATP in the electron transport chain.
Osmosis? - ANSWERS-The movement of water across a selectively permeable
membrane from an area of high water to an area of low water concentration.
, What is/purpose of glycocalyx? - ANSWERS-Is outside cell wall and sticky. capsule:
neatly organized which helps evade mucocytosis, slime layer: unorganized and
loose. Capsules prevent phagocytosis and is significantly larger that cell.
Gram positive cell wall - ANSWERS-Has thick peptidoglycan, teichoic acids (which
is only found in Gram positive cells), and are more simple. Has 2 ring basal body in
flagella, disrupted by lysozymes, PENICILLIN SENSITIVE.
Cocci shape - ANSWERS-Spherical, round
What is a smear ? - ANSWERS-A thin film of a solution of microbes on a slide. Is
usually fixed to attach the microbes to the slide in to kill the microbes., stains the
background and not the cell structures.
For each Electron transport chain with carbohydrate catabolism (aerobic
respiration) - ANSWERS-Most of ATP is produced this way
Summary of Krebs cycle (TCA cycle) - ANSWERS-For each acetylCoA oxidized, TCA
cycle generates: 2 CO2, 3 NADH, 1 FADH2, 1 GTP. There are 2 turns off the TCA
cycle per 1 molecule of glucose.
Eukaryotic Glycocalyx - ANSWERS-Carbohydrates extending from animal plasma
membrane. Bonded to proteins and lipids in membrane
Non-competitive or allosteric inhibition - ANSWERS-Inhibitor binds at a different
site than the substrate (allosteric site). Changes shape of enzyme.
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