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BIOL 266 Final Exam 5 docx Concordia University
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BIOL 266 Final Exam 5 docx 2024-2025 Concordia University
,Exam 1:
MULTIPLE CHOICES:
1. You have isolated a mutant line of mammalian cells deficient in emerin.
This mutant line is expected to be impaired in:
A. Export of 40S and 60S ribosomal subunits from the nucleus
B. Ribosome assembly in the nucleolus
C. Transcription of some genes in certain tissues
D. Nuclear import of lamins
E. Attachment of lamins to the terminal ring of the nuclear basket
2. Some of the enzymes inserted into the outer mitochondrial
membrane convert fatty acids into forms that are subsequently
metabolized in the mitochondrial matrix. Lack of these enzymes is
expected to:
A. Completely block the synthesis of ATP in mitochondria
B. Completely abolish the formation of acetyl-CoA in the mitochondria
C. Completely eliminate the flow of electrons through the mitochondrial
electron transport chain
D. Completely shut down the formation of CO2 in mitochondria
E. All of the above
F. None of the above
3. ATP synthesis in the thylakoid membrane catalyzes the synthesis of
ATP. This newly formed ATP is released to:
A. The intermembrane space between the inner and outer membranes of
the chloroplast
B. The stroma of the chloroplast
C. The thylakoid lumen
D. The space outside of the chloroplast
E. All of the above
F. None of the above
4. Plant cells synthesize hemicelluloses and pectins in the Golgi
apparatus. Lack of the enzymes that catalyze the synthesis of these two
compounds is expected to:
A. Prevent the retrograde vesicular transport of proteins from the Golgi to the ER
B. Abolish vesicular transport of proteins from the Golgi to the lysosome
C. Change the composition of the cell wall
D. Impair the vesicular transport of proteins from the Golgi to the plasma membrane
E. All of the above
F. None of the above
, 5. Lysosomes contain a proton ATPase. Lack of this enzyme is expected to:
A. Stimulate the ability of cells to digest small molecules taken up from outside
the cell by endocytosis
B. Promote autophagy
C. Activate phagocytosis
D. Increase pH in the lumen of the lysosome
E. Decrease pH in the lumen of the lysosome
F. None of the above
6. The nucleolus is:
A. The site for phospholipid synthesis
B. The most prominent membrane-bound organelle in the nucleus
C. The site of protein synthesis
D. The assembly centre for chromatin
E. The assembly centre for ribosomes
F. The site of sphingolipid synthesis
FROM PRACTICE PROBLEMS
7. In a typical mammalian cell, primary lysosomes digest:
A. Aged or defective mitochondria
B. Bacteria taken up from outside the cell by phagocytosis
C. Aged or defective peroxisomes
D. The debris of dead cells taken up from outside the cell by phagocytosis
E. All of the above
F. None of the above
8. The receptor for the thyroid hormone thyroxine:
A. Forms a dimer only in the presence of thyroxine
B. Is located on the outer surface of the plasma membrane
C. Is always located in the nucleolus
D. Is located in the cytosol
E. In the absence of thyroxine acts as a repressor of transcription of
thyroxine- inducible genes
F. Is transported into the nucleus only after it binds to thyroxine
9. The densities of organelles are listed below:
Golgi = 1.14 g/cm3, the density of 20% sucrose; lysosome = 1.10 g/cm3, the
density of 10% sucrose; mitochondria = 1.18 g/cm3, the density of 30%
sucrose; peroxisomes = 1.23 g/cm3, the density of 42% sucrose; plasma
membrane vesicles = 1.12 g/cm3, the density of 15% sucrose; nuclei = 1.28
g/cm3, the density of 47% sucrose; vesicles of the rough ER = 1.20 g/cm3, the
density of 35% sucrose.
Peroxisomes and nuclei can be separated from each other using:
A. Centrifugation of a cell homogenate in an isotonic buffer at 20 000 x g
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